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Related Experiment Video

Updated: May 13, 2026

Droplet-based Cytotoxicity Assay to Assess Chimeric Antigen Receptor T cells at the Single-cell Level
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Droplet-based Cytotoxicity Assay to Assess Chimeric Antigen Receptor T cells at the Single-cell Level

Published on: March 14, 2025

Evaluating Multimodal Functionality of Chimeric Antigen Receptor T Cells at Single-Cell Resolution through

Julia Franz1, Fabian Freitag1, Patrick Ho1

  • 1Lehrstuhl für Zelluläre Immuntherapie, Medizinische Klinik und Poliklinik II, Universitätsklinikum Würzburg.

Journal of Visualized Experiments : Jove
|May 11, 2026
PubMed
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This study introduces an optofluidics method to assess individual chimeric antigen receptor (CAR)-T cells for improved cancer immunotherapy. This approach enables the isolation of highly functional CAR-T cells, potentially enhancing treatment durability.

Area of Science:

  • Immunology
  • Cell Therapy
  • Biotechnology

Background:

  • Adoptive T-cell therapy, specifically chimeric antigen receptor (CAR)-T cell therapy, shows promise for hematological malignancies.
  • Variability in clinical outcomes is observed, with polyfunctional T cells correlating with durable remission.
  • Current CAR-T cell product evaluations use bulk measurements, hindering the study of functional subpopulations.

Purpose of the Study:

  • To develop and demonstrate a workflow for evaluating individual CAR-T cell functionality.
  • To enable the isolation of highly functional CAR-T cell subpopulations for next-generation therapy design.

Main Methods:

  • Utilized an optofluidics platform to assess individual CAR-T cell cytokine secretion and CD137 expression.
  • Integrated cytotoxic activity assessment as an optional component.

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A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells
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A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells

Published on: November 12, 2019

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Last Updated: May 13, 2026

Droplet-based Cytotoxicity Assay to Assess Chimeric Antigen Receptor T cells at the Single-cell Level
08:09

Droplet-based Cytotoxicity Assay to Assess Chimeric Antigen Receptor T cells at the Single-cell Level

Published on: March 14, 2025

A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells
08:46

A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells

Published on: November 12, 2019

  • Enabled isolation of CAR-T cells with multimodal functionality.

    • Main Results:

    • Demonstrated a workflow for single-cell analysis of CAR-T cell function, including cytokine profiles and activation markers.
    • Showcased the ability to identify and isolate CAR-T cells with superior polyfunctional characteristics.
    • Established a method to link pre-infusion CAR-T cell quality to potential therapeutic efficacy.

    Conclusions:

    • The optofluidics platform provides a powerful tool for detailed characterization of CAR-T cell products.
    • Isolation of highly functional CAR-T cells can inform the development of more effective and durable cancer immunotherapies.
    • This approach facilitates the design of next-generation CAR-T cell therapies with improved clinical outcomes.