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Updated: Jul 1, 2026

Manufacturing Chimeric Antigen Receptor (CAR) T Cells for Adoptive Immunotherapy

Manufacturing Chimeric Antigen Receptor (CAR) T Cells for Adoptive Immunotherapy

Published on: December 17, 2019

Standardized Flow Cytometry Assay Using Dried Recombinant Antibody Panels for CAR T Cell Characterization.

Annika Ridzal¹, Anja Brauchle², Desiré Missing²

¹Miltenyi Biotec; annikag@miltenyi.com.

Journal of Visualized Experiments : Jove

|June 29, 2026

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This summary is machine-generated.

Dried recombinant antibody panels streamline Chimeric Antigen Receptor T (CAR T) cell analysis via flow cytometry. This ready-to-use format reduces preparation steps and operator variability for more consistent critical quality attribute assessment.

Area of Science:

Immunology
Biotechnology
Analytical Chemistry

Background:

Flow cytometry is essential for characterizing Chimeric Antigen Receptor T (CAR T) cells, assessing critical quality attributes like viability, phenotype, and transduction efficiency.
Conventional liquid reagent workflows involve multiple preparation steps, leading to potential variability and operator-dependent inconsistencies.
Recombinant antibody technology offers enhanced specificity and reduced lot-to-lot variability compared to traditional antibodies.

Purpose of the Study:

To present a streamlined, step-by-step workflow for CAR T cell analysis using preformulated dried recombinant antibody panels.
To demonstrate the practical application of dried antibody panels in multiparametric CAR T cell characterization.
To compare the hands-on time and efficiency of dried reagent panels against conventional liquid reagent workflows.

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Main Methods:

Development and validation of a protocol for CAR T cell staining using dried recombinant antibody panels.
Flow cytometry data acquisition using the optimized staining protocol.
Analysis of CAR T cell critical quality attributes, including viability, phenotype, and transduction efficiency.
Comparison of hands-on time and workflow efficiency with traditional liquid reagent methods.

Main Results:

A practical, step-by-step workflow for CAR T cell analysis using dried recombinant antibody panels was established.
Dried panels simplified reagent handling and reduced preparation steps, leading to decreased hands-on time.
Representative staining data demonstrated the utility of dried panels for multiparametric CAR T cell characterization.
Reduced lot-to-lot variability was observed due to the use of recombinant antibody technology.

Conclusions:

Dried recombinant antibody panels offer a practical and efficient alternative to conventional liquid reagents for CAR T cell analysis.
This approach enhances consistency and reduces operator variability in flow cytometry workflows.
The presented framework facilitates the implementation of dried antibody panels for streamlined multiparametric characterization of CAR T cells.