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Updated: May 14, 2026

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Murrayone: A Promising New Fungicide Destroying the Oxidative Homeostasis and Cell Membrane Integrity of Botrytis

Wanyun Zhu1,2, Yao Yu1,2, Xinru Li1,2,3

  • 1Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China.

Journal of Agricultural and Food Chemistry
|May 12, 2026
PubMed

Insights

Murrayone effectively combats the destructive plant pathogen Botrytis cinerea by damaging cell membranes and disrupting oxidative balance. This natural compound shows promise as a safe and sustainable antifungal agent for agriculture.

Area of Science:

  • Agricultural Science
  • Plant Pathology
  • Mycology

Background:

  • Botrytis cinerea causes significant global economic losses in agriculture.
  • Previous studies identified Murrayone as a potent natural antifungal agent against B. cinerea.

Purpose of the Study:

  • To investigate the mechanism of action of Murrayone against B. cinerea.
  • To evaluate the safety and efficacy of Murrayone as a sustainable antifungal strategy.

Main Methods:

  • Cellular morphology, sporulation, and spore germination assays were performed.
  • Transcriptome analysis was used to identify molecular pathways affected by Murrayone.
  • Reactive oxygen species (ROS) accumulation, propidium iodide (PI) staining, and mycelial dry weight were assessed.

Main Results:

  • Murrayone induced severe cellular damage, inhibited sporulation and germination, and reduced disease severity in blueberries.
  • Transcriptome analysis indicated disruption of oxidative homeostasis and cell membrane damage.
  • Murrayone demonstrated safety in seed germination assays.

Conclusions:

  • Murrayone exhibits multitarget and multipathway antifungal effects against B. cinerea.
  • The findings provide a theoretical basis for developing Murrayone as a sustainable agricultural antifungal agent.
Keywords:
Botrytis cinereaMurrayoneantifungal mechanismcell membrane integrityoxidative stresstranscriptomics

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