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Green Synthesis, Characterization, Encapsulation, and Measurement of the Release Potential of Novel Alkali Lignin Micro-/Submicron Particles
Published on: March 1, 2024
Vital Roles of A-Type Linkage and Polymerization Degree in Governing Proanthocyanidin Interaction and
Yun Li1, Chibuike C Udenigwe2,3, Carine Le Bourvellec4
1Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
Abstract:
This study investigated the effects of proanthocyanidins (PC) with different degree of polymerization (DP) and A-type linkage on the structural and functional properties of litchi thaumatin-like protein (LcTLP). PC were extracted from litchi shells (S) and pulp (P) using a sequential solvent extraction with methanol (M) and acetone (A), and were designated as SMPC (DP 1.9), SAPC (DP 2.7), and PAPC (DP 12.6), respectively. Results showed that proanthocyanidin structural features strongly dictated their binding modes with LcTLP. SMPC and SAPC with high A-type linkage ratios (67.8% and 41.7%) inserted into LcTLP hydrophobic cavity, increasing α-helix content. PAPC showed the highest affinity (Ka = 283.7 L/mol) with LcTLP. Thermodynamic parameters indicated hydrogen bonding as dominant, reflecting an enthalpy-driven process. In RAW264.7 cells, PAPC more effectively suppressed LcTLP-induced NO, TNF-α, and IL-6 secretion by coating LcTLP surface. However, oligomeric proanthocyanidins occupied LcTLP active site by binding to the key residues, including GLU84 and TYR85.
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