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Green Extraction Strategies for Orange Peel Dust Valorization with Enhanced Bioactive Potential.

Isidora Vlaović1, Slađana Krivošija1, Vanja Travičić1

  • 1Faculty of Technology Novi Sad, University of Novi Sad, Boulevard Cara Lazara 1, 21000 Novi Sad, Serbia.

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Summary

Orange peel dust (OPD) is a valuable industrial byproduct. Green extraction methods like PEE and UAE effectively isolate bioactive compounds, demonstrating OPD

Keywords:
bioactive recoverycircular bioeconomycitrus by-productsenzyme inhibitionpressurized ethanol extractionsubcritical water extractionsupercritical CO2ultrasound-assisted extraction

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Area of Science:

  • Agro-industrial byproducts valorization
  • Green chemistry and sustainable processing
  • Food science and nutraceuticals

Background:

  • Orange peel dust (OPD) is an abundant industrial byproduct from citrus processing, largely underutilized.
  • OPD possesses a rich bioactive composition with potential applications.
  • Sustainable valorization of OPD aligns with circular economy principles.

Purpose of the Study:

  • To compare four green extraction technologies for valorizing orange peel dust (OPD).
  • To evaluate antioxidant, antidiabetic, and antimicrobial properties of extracts obtained from OPD.
  • To analyze the sugar composition of OPD extracts under different green extraction conditions.

Main Methods:

  • Subcritical water extraction (SWE), pressurized ethanol extraction (PEE), ultrasound-assisted extraction (UAE), and sequential supercritical CO2-UAE (Sc-CO2-UAE) were employed.
  • Antioxidant activity was assessed using DPPH, ABTS, and reducing power assays.
  • Antimicrobial activity against specific fungi and bacteria, and α-amylase inhibitory activity were evaluated.

Main Results:

  • PEE at 220 °C yielded extracts with the highest radical-scavenging activity.
  • UAE demonstrated broad antifungal activity and selective antibacterial effects.
  • Sequential Sc-CO2-UAE pretreatment followed by UAE resulted in the highest α-amylase inhibitory activity.
  • Thermal extraction enhanced carbohydrate hydrolysis, while UAE and Sc-CO2-UAE preserved structural sugars.

Conclusions:

  • Green extraction technologies offer superior performance compared to conventional maceration for OPD valorization.
  • OPD is a valuable matrix for sustainable extraction of bioactive compounds.
  • These findings support the circular economy in the citrus processing industry.