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Related Concept Videos

Formation of Muscle Fibers from Myoblasts01:13

Formation of Muscle Fibers from Myoblasts

De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription factors...

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Studying Muscle Transcriptional Dynamics at Single-molecule Scales in Drosophila
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Single-Cell Transcriptomic Analysis Reveals Multicellular Coordination and Signaling Rewiring During Fetal Goat

Shiyao Han1, Shengcan Xie1, Fenfen Jiang1

  • 1Shaanxi Centre of Stem Cells Engineering & Technology, Key Laboratory of Livestock Biology, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.

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|May 13, 2026
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Summary

This study maps fetal skeletal muscle development in goats, identifying key cell types and signaling pathways. It reveals how stromal, vascular, and immune cells orchestrate muscle maturation from progenitors to mature fibers.

Keywords:
goatmusclesingle cell RNA-seqskeletal muscle

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Published on: April 30, 2014

Area of Science:

  • Developmental biology
  • Muscle biology
  • Single-cell genomics

Background:

  • Fetal skeletal muscle development is complex, involving multiple cell types.
  • Understanding tissue maturation requires detailed cellular and molecular insights.

Purpose of the Study:

  • To create a high-resolution single-cell atlas of fetal goat skeletal muscle.
  • To elucidate the cellular dynamics and molecular programs governing muscle maturation.

Main Methods:

  • Single-cell RNA sequencing (scRNA-seq) of fetal goat skeletal muscle.
  • Trajectory inference, transcription factor activity, and cell-cell communication analysis.
  • Bioinformatic analysis including KEGG and Gene Ontology (GO) enrichment.

Main Results:

  • Identified distinct cell populations: mesenchymal progenitors, fibro-adipogenic progenitors (FAPs), myocytes, and mature fibers.
  • Traced a myogenic maturation continuum, highlighting extracellular matrix remodeling and sarcomere assembly.
  • Revealed stage-specific signaling pathways (ErbB, Hedgehog, Hippo) and transcription factor dynamics (E2F4/5, HAND2, CEBPB, ELK1).

Conclusions:

  • Defined the cellular ecosystem supporting myogenic progression during fetal development.
  • Established the transcriptional and signaling framework orchestrating skeletal muscle maturation.
  • Showcased dynamic intercellular communication shifts during tissue development.