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A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
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Single-Cell Selective Retrieval Method Using Cone-Shaped Light-Responsive Gas-Generating Polymer Microscaffold Array

Hidetaka Ueno1, Yoshinori Akagi2, Shohei Yamamura3

  • 1Center for Advanced Medical Engineering Research & Development (CAMED), Kobe University, 1-5-1 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Hyogo, Japan.

Sensors (Basel, Switzerland)
|May 13, 2026
PubMed
Summary

Researchers developed a novel method for single-cell retrieval using light-responsive polymers. This technique enables quick and gentle isolation of specific cells, crucial for cancer diagnosis and cell function studies.

Keywords:
cell chipheterogeneitylight-responsive gas-generating polymersingle-cell analysissingle-cell retrieval

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Live-cell Imaging of Single-Cell Arrays (LISCA) - a Versatile Technique to Quantify Cellular Kinetics

Published on: March 18, 2021

Area of Science:

  • Biotechnology
  • Materials Science
  • Cell Biology

Background:

  • Single-cell retrieval is vital for cellular function studies and early cancer detection.
  • Existing microcapillary methods struggle with speed, cell adhesion, and preventing cell damage.

Purpose of the Study:

  • To develop a simple, rapid, and non-damaging method for selective single-cell retrieval.
  • To utilize a novel light-responsive gas-generating polymer (LGP) microscaffold array chip for cell isolation.

Main Methods:

  • Fabrication of a cone-shaped LGP microscaffold array chip without specialized equipment.
  • Adhesion and arrangement of human cervical cancer cells on the LGP microscaffolds.
  • Release of single cells using nitrogen gas bubbles generated by UVA light irradiation.

Main Results:

  • Single cells successfully adhered and arranged on up to 73.3% of the microscaffolds.
  • Over 90% of retrieved cells maintained viability, adhered, spread, and were culturable for over 24 hours.
  • The method demonstrated high selectivity and minimal cell damage.

Conclusions:

  • The proposed LGP microscaffold chip offers a simple and quick solution for single-cell retrieval.
  • This technique is compatible with standard inverted fluorescence microscopes.
  • The method holds promise for applications in cancer diagnostics and cell biology research.