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Updated: May 14, 2026

Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay
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Development of a Highly Sensitive Analytical System for Measuring 17β-Estradiol Using Fluorescent Molecular Probes.

Yoshio Suzuki1

  • 1Health and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba 305-8566, Ibaraki, Japan.

Sensors (Basel, Switzerland)
|May 13, 2026
PubMed
Summary

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A new fluorescence assay using aptamers allows rapid and sensitive detection of 17β-estradiol (estrogen) in saliva. This simple method aids early diagnosis and treatment of women's health conditions.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Accurate measurement of 17β-estradiol is crucial for diagnosing and treating various women's health conditions.
  • Current detection methods can be complex and time-consuming, hindering early diagnosis.

Purpose of the Study:

  • To develop a rapid, sensitive, and selective fluorescence-based assay for 17β-estradiol detection.
  • To create a user-friendly system for estrogen measurement in biological samples.

Main Methods:

  • A nucleic acid aptamer labeled with a fluorescent dye was designed for 17β-estradiol detection.
  • The assay utilizes aptamer conformational changes upon estrogen binding to induce a fluorescence signal.
  • A compact desktop fluorescence detector was developed for simplified measurements.
Keywords:
aptamerestrogenfluorescencemolecular probesportable device

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Last Updated: May 14, 2026

Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay
06:07

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Published on: June 7, 2020

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH
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Main Results:

  • The assay provides rapid detection (within 30 min) with a low limit of detection (0.2 pg/mL) and a wide linear dynamic range (1-1000 pg/mL).
  • High selectivity for 17β-estradiol was observed against related compounds.
  • Successful application to human saliva samples demonstrated high sensitivity, precision (recoveries 98.8%, CV <3.0%).

Conclusions:

  • The developed fluorescence aptasensor system offers a simple, practical, and sensitive platform for estrogen detection.
  • This method has significant potential for clinical diagnostics and research applications in women's health.