Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spatiotemporally Asynchronous Dual-Modality Probe: Real-Time and Accurate Monitoring of Neutrophil Recruitment in Phototherapy Prognosis.

Journal of the American Chemical Society·2026
Same author

Engineering Organelle-Gated Reporters for Imaging Subcellular Enzyme Activity in Living Cells.

Journal of the American Chemical Society·2026
Same author

Hypoxia-induced ADAM8⁺ macrophages and FAP⁺ fibroblasts form an extracellular matrix-remodeling niche at the tumor boundary in HCC.

Cancer immunology research·2026
Same author

Repurposing methylene blue as activatable prodrugs for precise disease theranostics.

Chemical communications (Cambridge, England)·2026
Same author

RRM2 Promotes the Progression of Intrahepatic Cholangiocarcinoma by Inhibiting Ferroptosis Through the HIF-1α Pathway.

Liver international : official journal of the International Association for the Study of the Liver·2026
Same author

Resection combined with hyperthermic intraperitoneal chemotherapy in the treatment of spontaneously ruptured hepatocellular carcinoma: a multicenter retrospective study.

Therapeutic advances in medical oncology·2026

Related Experiment Video

Updated: May 14, 2026

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment
13:01

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment

Published on: June 3, 2022

Synergistic Probe Combining Lysosome Anchoring and Tumor Defense System Targeting for Precise Tumor Visualization.

Yang Shen1,2, Jiawei Li2, Ruixi Yi2

  • 1Department of Hepatobiliary Surgery, Central Laboratory, Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha, P. R. China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|May 13, 2026
PubMed
Summary

Researchers developed a new optical probe that accurately images tumors by targeting lysosomes. This advanced imaging tool enhances precision in cancer surgery, reducing both missed and excessive resections.

Keywords:
activatable fluorescent probehigh‐precision imaginglysosomemolecular imagingtumor imaging

More Related Videos

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody
07:36

Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody

Published on: May 16, 2020

Related Experiment Videos

Last Updated: May 14, 2026

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment
13:01

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment

Published on: June 3, 2022

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody
07:36

Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody

Published on: May 16, 2020

Area of Science:

  • Biomedical Engineering
  • Molecular Imaging
  • Cancer Diagnostics

Background:

  • Accurate tumor imaging is critical for surgical decisions and patient outcomes.
  • Current optical molecular probes struggle with false positives and negatives, limiting clinical use.
  • Lysosomal abundance increases during tumor metabolic reprogramming, offering a potential imaging target.

Purpose of the Study:

  • To develop an activatable optical probe for high-precision tumor imaging.
  • To overcome limitations of existing probes in differentiating cancerous from healthy tissues.
  • To create a probe that aids intraoperative decision-making and improves surgical accuracy.

Main Methods:

  • Synthesized a lysosome-targeted, activatable optical probe (CN-D-GGT) by functionalizing a precursor (CN-D-NH2) with a gamma-glutamyltransferase substrate.
  • Leveraged lysosomal protonation for enhanced probe retention and single-cell resolution.
  • Utilized multi-modal imaging capabilities, including fluorescence and photoacoustic signals.

Main Results:

  • The probe (CN-D-GGT) demonstrated significant changes in optical signals upon activation.
  • Achieved high specificity, distinguishing cancer cells in co-culture (approx. 6-fold) and tumors from surrounding tissues (T/N or T/I ratios > 3.5).
  • Successfully differentiated cancerous from adjacent tissues in clinical samples.

Conclusions:

  • Developed a novel optical probe for accurate tumor visualization in complex pathological environments.
  • The probe's design enables precise imaging, potentially assisting in intraoperative tumor resection.
  • This technology has the potential to reduce incomplete or overly aggressive surgical interventions.