Related Experiment Video
Updated: May 15, 2026

Functionalized Spirocyclic Heterocycle Synthesis and Cytotoxicity Assay
Published on: February 9, 2021
Synthesis and mechanistic insights of carbazole-based inhibitors targeting FTO by spectroscopic characterization and
Miao Zhang1, Leyao Chen1, Xiaoyu Chang2
1College of Chemistry, Pingyuan Laboratory (Zhengzhou), Zhengzhou UniversityUniversity, Zhengzhou 450001, China.
Abstract:
FTO (Fat mass and obesity-associated protein) is an RNA demethylase crucial for epitranscriptomic regulation, catalyzing the removal of N6-methyladenosine (m6A) modifications. We designed a series of carbazole derivatives and identified compound K27 as a promising FTO inhibitor, exhibiting an IC50 value of 35.58 ± 5.23 μM. Spectroscopic analyses confirmed the binding of K27 to FTO and revealed ligand-induced changes in the protein microenvironment. Moreover, molecular docking and 300 ns molecular dynamics simulations demonstrated stable binding of K27 within the FTO active site. Collectively, these results highlight K27 as a promising lead scaffold for developing FTO inhibitors.
More Related Videos
06:34Synthesis of Antiviral Tetrahydrocarbazole Derivatives by Photochemical and Acid-catalyzed C-H Functionalization via Intermediate Peroxides (CHIPS)
Published on: June 20, 2014
04:51Synthesis of Triazole and Tetrazole-Functionalized Zr-Based Metal-Organic Frameworks Through Post-Synthetic Ligand Exchange
Published on: June 23, 2023
Related Concept Videos
Induced-fit Model
Enzymes exhibit substrate specificity, meaning that they can only bind to certain substrates. This is mainly determined by the shape and chemical characteristics of...
Protein-Drug Binding: Mechanism and Kinetics
Various forces drive these interactions, including hydrogen bonds, hydrophobic interactions, ionic bonds, electrostatic interactions, and van der Waals forces. These bonds enable drugs to bind to specific sites on proteins,...
Inhibitors of Bacterial DNA Synthesis