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Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...

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Related Experiment Video

Updated: May 15, 2026

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
12:55

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

In Vitro Selection of Antibodies by Ribosome Display.

He Huang1, Xinran Gao2, Guangbo Kang2

  • 1Department of Biochemical Engineering, School of Chemical Engineering & Technology, Tianjin University, Tianjin, PR China. huang@tju.edu.cn.

Methods in Molecular Biology (Clifton, N.J.)
|May 13, 2026
PubMed
Summary
This summary is machine-generated.

Ribosome display is a powerful cell-free system for selecting high-performance protein binders. This study details methods for constructing and characterizing ribosome-displayed nanoantibody libraries for specific screening.

Keywords:
Display technologiesIn vitro selectionNanobodyRibosome display

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Last Updated: May 15, 2026

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Area of Science:

  • Biotechnology and Molecular Engineering
  • Immunology and Protein Engineering

Background:

  • Ribosome display is a well-established, powerful in vitro selection tool for engineering high-performance protein binders.
  • This cell-free display system has over 20 years of development and offers unique advantages compared to other display techniques.
  • It has been successfully used for selecting functional and specific binders against various antigens.

Purpose of the Study:

  • To describe methods for constructing ribosome-displayed natural nanoantibody libraries.
  • To detail the characterization of these libraries.
  • To enable specific nanoantibody screening using this platform.

Main Methods:

  • Construction of natural nanoantibody libraries for ribosome display.
  • Characterization techniques for assessing library diversity and functionality.
  • Application of the platform for specific nanoantibody screening.

Main Results:

  • Successful construction and characterization of ribosome-displayed natural nanoantibody libraries.
  • Demonstration of the platform's capability for specific nanoantibody screening.
  • Validation of ribosome display as an effective tool for generating nanoantibody binders.

Conclusions:

  • Ribosome display is a robust and versatile platform for engineering nanoantibody binders.
  • The described methods facilitate the generation of diverse nanoantibody libraries for targeted antigen selection.
  • This approach holds significant promise for antibody discovery and therapeutic development.