Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Spatiotemporal Evolution of Carbon Sources/Sinks and Analysis of Multi-factor Driving Mechanisms in the Weihe River Basin].

Huan jing ke xue= Huanjing kexue·2026
Same author

MiR-26b-5p Predicts the Severity of Crohn's Disease and the Degree of Malnutrition.

Folia biologica·2026
Same author

Dual-Interface Nanopore Array Sensor Revealing the Synergistic Oscillations of Lactate Efflux in Cancer Cells.

Angewandte Chemie (International ed. in English)·2026
Same author

Analysis of Intraoperative Frozen Section Findings of Thyroid Lesions in Xizang.

Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae·2026
Same author

Molecular epidemiology of <i>Babesia microti</i> in southern Zhejiang: an integrated survey of humans, rodent reservoirs, and tick vectors (2020-2023).

Frontiers in microbiology·2026
Same author

Pyroptosis Plays a Key Role Through Macrophages in Primary Biliary Cholangitis of Mice.

BioMed research international·2026

Related Experiment Video

Updated: May 15, 2026

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics
10:50

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Published on: July 16, 2018

Nanosensor Quantifying Lysosomal Glycosidase Secretion from Single Living Cells.

Yu-Ting Qi1, Ru-Yan Zha1, Xiao-Xiao Wang1

  • 1College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China.

Journal of the American Chemical Society
|May 14, 2026
PubMed
Summary

Researchers developed a new method to track lysosomal enzyme secretion in living immune cells. This technique uses artificial substrates and sensors to quantify glycosidase release, aiding immune homeostasis research.

More Related Videos

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Live-cell Imaging of Lysosomal Membrane Permeabilization During Necroptosis
05:30

Live-cell Imaging of Lysosomal Membrane Permeabilization During Necroptosis

Published on: November 14, 2025

Related Experiment Videos

Last Updated: May 15, 2026

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics
10:50

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Published on: July 16, 2018

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Live-cell Imaging of Lysosomal Membrane Permeabilization During Necroptosis
05:30

Live-cell Imaging of Lysosomal Membrane Permeabilization During Necroptosis

Published on: November 14, 2025

Area of Science:

  • Immunology
  • Cell Biology
  • Biomedical Engineering

Background:

  • Secretory lysosomes are vital for immune cell function, regulating both degradation and secretion.
  • Disruptions in lysosomal secretion can cause immune imbalances and health issues.
  • Existing methods like immunostaining lack real-time, quantitative analysis and compromise cell viability.

Purpose of the Study:

  • To develop an innovative method for real-time, quantitative monitoring of lysosomal enzyme secretion from single living immune cells.
  • To overcome limitations of current immunostaining techniques.
  • To investigate the dynamics of secretory lysosomes in maintaining immune homeostasis.

Main Methods:

  • Combined artificial substrates with hybrid nanowire-nanopipette sensors.
  • Induced glycosidase secretion in macrophages via frustrated phagocytosis.
  • Quantified secreted glycosidases by detecting electroactive aminophenol released from substrate hydrolysis using nanowire electrodes.

Main Results:

  • Successfully quantified lysosomal glycosidase secretion from individual living macrophages.
  • Demonstrated a novel approach for real-time, quantitative assessment of enzyme secretion.
  • Provided insights into the dynamic role of secretory lysosomes in immune regulation.

Conclusions:

  • Developed a versatile methodology for monitoring lysosomal enzyme secretion in single living macrophages.
  • Highlighted the importance of secretory lysosomes in maintaining immune homeostasis.
  • Established a new tool for studying immune cell function and related diseases.