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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Related Experiment Video

Updated: May 16, 2026

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

Recent Advances in Top-Down Proteomics for Single-Cell Research.

Jake A Melby1, Pei Su2, Fabio P Gomes3

  • 1Integrated Bioanalysis, Clinical Pharmacology and Safety Sciences, R&D, AstraZeneca, Gaithersburg, Maryland, USA.

Mass Spectrometry Reviews
|May 14, 2026
PubMed
Summary

Top-down proteomics (TDP) now enables detailed analysis of protein variants (proteoforms) in single cells (SCs). Advances in mass spectrometry and computational methods are driving this field forward for SC research.

Keywords:
proteoformssingle cellstop‐down proteomics

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Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
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Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Published on: December 9, 2022

Related Experiment Videos

Last Updated: May 16, 2026

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
06:13

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Published on: December 9, 2022

Area of Science:

  • Biochemistry
  • Proteomics
  • Cell Biology

Background:

  • Individual cells possess unique molecular signatures, including biologically significant proteoforms.
  • Single-cell (SC) omics provide deep insights into cellular heterogeneity and function.
  • Characterizing proteoforms within individual cells remains a significant challenge.

Purpose of the Study:

  • To review the importance of top-down proteomics (TDP) for profiling proteoforms in SCs.
  • To highlight recent advancements in TDP techniques applicable to SC analysis.
  • To discuss the biological relevance and potential of MS-based proteoform analysis in SCs.

Main Methods:

  • Mass spectrometry (MS)-based proteomics, specifically top-down proteomics (TDP).
  • Review of recent advances in sample processing, separation techniques, and MS instrumentation for SC analysis.
  • Overview of computational approaches for analyzing proteoforms in individual cells.

Main Results:

  • TDP has demonstrated proficiency in characterizing proteoforms within single cells.
  • Recent technological and computational improvements have overcome previous barriers in SC proteoform analysis.
  • New avenues for large-scale SC proteoform studies are emerging.

Conclusions:

  • TDP is a crucial technology for understanding proteoform heterogeneity in SCs.
  • Ongoing advancements are expanding the capabilities and applications of SC-TDP.
  • Further development is needed to fully realize the potential of MS-based proteoform analysis in SC research.