Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fast Reactions01:27

Fast Reactions

Fast reactions occurring in times shorter than the time needed to mix reactants pose a unique challenge for investigation. In a liquid-phase continuous-flow system, reactants A and B are swiftly pushed into the mixing chamber, where mixing occurs within 1 ms. The reaction mixture then flows through an observation tube, and one measures light absorption to determine species concentrations at various points of the tube. This method is most appropriate when relatively large volumes of reactants...
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other axis.
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
Fast Decoupled and DC Powerflow01:24

Fast Decoupled and DC Powerflow

The fast decoupled power flow method addresses contingencies in power system operations, such as generator outages or transmission line failures. This method provides quick power flow solutions, essential for real-time system adjustments. Fast decoupled power flow algorithms simplify the Jacobian matrix by neglecting certain elements, leading to two sets of decoupled equations:
Assessment of Diffusion and Perfusion01:17

Assessment of Diffusion and Perfusion

Understanding and evaluating diffusion and perfusion is critical in assessing a patient's respiratory and circulatory health. These processes play key roles in maintaining the body's internal environment, ensuring that tissues receive adequate oxygen while waste products are efficiently removed.
The Role of Diffusion in Respiration
Diffusion is the process by which molecules move from an area of higher concentration to an area of lower concentration. In the respiratory system, this principle...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Robust single-scan ultraselective NMR.

Chemical communications (Cambridge, England)·2026
Same author

Multi-Solvent Suppression Ultrafast 2D COSY for High-Throughput Wine Screening.

Magnetic resonance in chemistry : MRC·2026
Same author

Cellulose Paper-Based Adsorbent of Heavy Metals in Water Fabricated by One-Step Ultrafast Ligation of Diazofluorene.

ACS omega·2025
Same author

Analytical Comparison of Two Quantitative HSQC Methods for the Absolute Quantitation of Metabolites.

Magnetic resonance in chemistry : MRC·2025
Same author

Evaluation of pure shift NMR methods for the analysis of complex metabolite mixtures with a benchtop NMR spectrometer.

Analytical methods : advancing methods and applications·2025
Same author

Hyperpolarized <sup>13</sup>C NMR Metabolomics of Urine Samples at Natural Abundance Applied to Chronic Kidney Disease.

Journal of the American Chemical Society·2024
Same journal

Aptamer-powered surveillance of SARS-CoV-3.

Chemical communications (Cambridge, England)·2026
Same journal

Does aurophilicity exist beyond the solid state?

Chemical communications (Cambridge, England)·2026
Same journal

Pressure-induced emission enhancement in 2-(anthracen-9-yl)-9<i>H</i>-thioxanthen-9-one crystals with π-π stacked thioxanthone dimers.

Chemical communications (Cambridge, England)·2026
Same journal

A Co-peptoid electrocatalyst for nitrite reduction that enables selective production of ammonia.

Chemical communications (Cambridge, England)·2026
Same journal

An AIE-based fluorescent probe for selective and sensitive detection of <i>N</i>-bromosuccinimide.

Chemical communications (Cambridge, England)·2026
Same journal

Harnessing the heteroatomic S/P coordination effects of FeCo dual-atomic catalysts for enhanced ORR performance.

Chemical communications (Cambridge, England)·2026
See all related articles

Related Experiment Video

Updated: May 16, 2026

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
15:10

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Published on: October 9, 2014

Fast and flow-compatible pseudo-3D diffusion NMR.

Yuliia Horbenko1, Patrick Giraudeau1, Francois-Xavier Felpin1

  • 1Nantes Université, CNRS, CEISAM, UMR, 6230, F-44000 Nantes, France. yuliia.horbenko@univ-nantes.fr.

Chemical Communications (Cambridge, England)
|May 14, 2026
PubMed
Summary
This summary is machine-generated.

Flow NMR and diffusion NMR are crucial for reaction monitoring and mixture analysis. A new flow-compensated DOSY ultrafast COSY experiment provides enhanced 3D spectral data in just 5 minutes, improving resolution.

More Related Videos

Blood Flow Imaging with Ultrafast Doppler
05:57

Blood Flow Imaging with Ultrafast Doppler

Published on: October 14, 2020

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA
12:05

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA

Published on: October 1, 2017

Related Experiment Videos

Last Updated: May 16, 2026

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
15:10

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Published on: October 9, 2014

Blood Flow Imaging with Ultrafast Doppler
05:57

Blood Flow Imaging with Ultrafast Doppler

Published on: October 14, 2020

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA
12:05

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA

Published on: October 1, 2017

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Nuclear Magnetic Resonance (NMR)

Background:

  • Flow NMR is essential for real-time reaction monitoring.
  • Diffusion NMR (DOSY) is valuable for analyzing complex mixtures.
  • Signal overlap in 2D diffusion NMR spectra limits resolution and analysis.

Purpose of the Study:

  • To address signal overlap in 2D diffusion NMR.
  • To improve spectral resolution and enable cross-correlation analysis.
  • To develop a rapid 3D NMR method for enhanced spectral information.

Main Methods:

  • Implementation of a flow-compensated DOSY ultrafast COSY experiment.
  • Acquisition of a 3D dataset combining diffusion and COSY information.
  • Utilizing ultrafast acquisition techniques to reduce experiment time.

Main Results:

  • Successfully acquired a 3D dataset in 5 minutes.
  • Demonstrated improved peak resolution by distributing spectral information over two chemical-shift dimensions.
  • Enabled cross-correlation analysis through the 3D spectral data.

Conclusions:

  • The developed method significantly enhances spectral resolution in diffusion NMR experiments.
  • Rapid 3D data acquisition in 5 minutes is achievable.
  • This technique offers a powerful tool for detailed analysis of complex chemical systems.