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Published on: September 9, 2012
Evaluating coagulation factor II deficiency using impulsive acoustic radiation force
José Francisco Silva Costa-Júnior1, João Carlos Machado2,3
1Postgraduate Program in Biomedical Engineering, Brazil University, Itaquera, SP, Brazil.
Biomedical Physics & Engineering Express
|May 14, 2026
Summary
This study introduces an ultrasonic system using impulsive acoustic radiation force (IARF) to detect clotting Factor II deficiency by measuring plasma stiffness. The system successfully distinguished between normal and deficient plasma, showing potential for diagnosing coagulation disorders.
Area of Science:
- Biomedical Engineering
- Materials Science
- Fluid Dynamics
Background:
- Impaired liver function and anticoagulant therapy can lead to deficiencies in crucial clotting factors like Factor II.
- Accurate detection of these deficiencies is vital for patient management and therapeutic monitoring.
- Existing diagnostic methods may require further refinement for speed and precision.
Purpose of the Study:
- To investigate the efficacy of an impulsive acoustic radiation force (IARF)-based ultrasonic system for assessing plasma viscoelasticity.
- To differentiate between normal human plasma and plasma deficient in coagulation Factor II.
- To evaluate the system's potential as a research tool for detecting coagulation factor deficiencies.
Main Methods:
- Utilized focused ultrasonic beams to induce microdisplacement in human plasma samples immersed with a solid sphere.
- Employed an ultrasonic transducer powered by a specific electrical signal (2.03406 MHz, 118 VPP, 1.249 Hz).
- Monitored microdisplacement using an SR-9000 board (4.89 MHz, pulse-echo mode) to calculate shear modulus (μ) and time-to-peak displacement (TPD).
Main Results:
- Significantly different shear modulus (μ) and time-to-peak displacement (TPD) values were observed between normal (μ: 300.11 ± 14.55 Pa) and Factor II-deficient plasma (μ: 75.53 ± 2.91 Pa).
- TPD ranged from 4.86 ± 0.28 ms to 6.97 ± 0.35 ms for control and deficient samples, respectively.
- The μ time-curve for prothrombin-deficient plasma showed a distinct pattern compared to normal plasma.
Conclusions:
- The IARF-based ultrasonic system demonstrates potential as a laboratory research tool for detecting viscoelastic changes in small plasma volumes.
- The system successfully distinguished between normal and Factor II-deficient plasma based on measured viscoelastic parameters.
- Further validation with larger cohorts and standardized protocols is necessary for clinical diagnostic applications.
