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Updated: May 16, 2026
Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults
Published on: April 22, 2022
3D Probe-Based Chemoproteomic Profiling Enables the Discovery of a Polyphenol Oxidase Catalyzing Oxidative
Zhuo-Zheng Xu1, Zheng Zhuang1, Hongling Yan1
1State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Innovation Research Institute of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, China.
Abstract:
Chemoproteomic profiling provides a complementary approach for linking natural products to their corresponding enzymes. A previously unrecognized polyphenol oxidase, SmPPOA, was identified from Salvia miltiorrhiza using a 3D carbene-immobilized salvianolic acid B. SmPPOA catalyzes oxidative lactonization of salvianolic acid B to generate salvianolic acid O via a cascade of oxidation and intramolecular Michael addition using the carboxylate anion as the Michael donor. This strategy can facilitate discovery of biosynthetic enzymes for plant natural products.
