Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A scientific approach to hemorrhage risk assessment of SSRIs/SNRIs utilizing the FAERS database.

Psychiatry research·2025

Same author

Evaluation of pitolisant, sodium oxybate, solriamfetol, and modafinil for the management of narcolepsy: a retrospective analysis of the FAERS database.

Frontiers in pharmacology·2024

Same author

Series or parallel toehold-mediated strand displacement and its application in circular RNA detection and logic gates.

Biosensors & bioelectronics·2023

Same author

Short divalent ethacrynic amides as pro-inhibitors of glutathione <i>S</i>-transferase isozyme Mu and potent sensitisers of cisplatin-resistant ovarian cancers.

Journal of enzyme inhibition and medicinal chemistry·2022

Same author

Tuning the specificity of DNA probes using bulge-loops for low-abundance SNV detection.

Biosensors & bioelectronics·2020

Same author

Method of damage location determination based on a neural network using a single fiber Bragg grating sensor.

Applied optics·2019

Search research articles

Related Experiment Video

Updated: May 17, 2026

Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents

Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents

Published on: August 16, 2024

Re-Engineering CRISPR-Cas12a into a Multimodal Biosensing Platform with Programmable Precursor crRNA.

Bangtian Xu¹, Shu Tian², Ning Yang²

¹Department of Pharmacy, Medical Sciences Research Center, University-Town Hospital of Chongqing Medical University, Chongqing 401331, China.

Analytical Chemistry

|May 15, 2026

Summary

Researchers developed programmable precursor CRISPR RNAs (pcRNAs) to enhance the CRISPR-Cas12a system for advanced molecular diagnostics. This innovation overcomes limitations, enabling direct RNA detection and improved specificity for next-generation biosensing applications.

More Related Videos

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

Related Experiment Videos

Last Updated: May 17, 2026

Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents

Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents

Published on: August 16, 2024

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

Area of Science:

Molecular Biology
Biotechnology
Diagnostics

Background:

CRISPR-Cas12a is a powerful diagnostic tool but has limitations for point-of-care applications.
Existing CRISPR-Cas12a systems require DNA activation and struggle with single-stranded DNA targets.
Low basal catalytic rates hinder direct detection in current CRISPR-Cas12a systems.

Purpose of the Study:

To engineer programmable precursor CRISPR RNAs (pcRNAs) to re-engineer Cas12a into a multimodal biosensing platform.
To overcome the inherent limitations of CRISPR-Cas12a for enhanced molecular diagnostics.
To establish a generalizable framework for next-generation intelligent and programmable molecular diagnostic systems.

Main Methods:

Engineered programmable precursor CRISPR RNAs (pcRNAs) to modify Cas12a functionality.
Integrated an autocatalytic circuit for signal amplification within the biosensing platform.
Validated the platform using synthetic circRNA detection and analysis in breast cancer cell lines.

Main Results:

The engineered Cas12a platform demonstrates programmable high specificity, distinguishing single-nucleotide DNA variants with selectivity up to 908.7.
Achieved high sensitivity for direct RNA detection, with a detection limit of 0.5 aM for synthetic circHER2 RNA.
Successfully validated quantitative assessment of circHER2 levels in complex cellular lysates from breast cancer cell lines.

Conclusions:

The developed pcRNA strategy breaks intrinsic functional constraints of Cas12a, creating a versatile biosensing platform.
The platform enables direct RNA detection without reverse transcription and offers enhanced specificity and sensitivity.
This work provides a generalizable framework for developing advanced, programmable molecular diagnostic and sensing systems.