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Updated: May 17, 2026
Detection of Disease-associated α-synuclein by Enhanced ELISA in the Brain of Transgenic Mice Overexpressing Human A53T Mutated α-synuclein
Published on: May 30, 2015
Protocol for using an ELISA to detect total α-synuclein levels in Drosophila melanogaster lines expressing human
Marco Sciortino1, Raquel Velazquez1, Haven Tillmon1
1Department of Cellular and Integrative Physiology, University of Texas San Antonio Health Science Center, Joe R. and Teresa Lozano Long School of Medicine, San Antonio, TX, USA.
Abstract:
In Parkinson's disease (PD), alpha-synuclein (α-syn) aggregation causes neuronal dysfunction and death, particularly of dopaminergic neurons, which is central to PD symptoms and progression. Here, we present a protocol for using a sandwich ELISA to quantify total α-syn levels in various Drosophila melanogaster genotypes expressing human α-syn point mutations associated with PD. We describe steps for aging flies, collecting fly heads, and preparing samples. We then detail procedures for ELISA setup and microplate reader analysis.
