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Updated: May 17, 2026
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Quantitative Analysis of Chromatin Proteomes in Disease
Published on: December 28, 2012
Nuclear Proteome Map of Mouse Heart Chambers
Seyed Sadegh Eslami1, Alin Rai2, Haoyun Fang3
1Baker Heart and Diabetes Institute, Melbourne, Victoria, Australia; Baker Department of Cardiovascular Research, Translation and Implementation La Trobe University, Melbourne, Victoria, Australia.
Molecular & Cellular Proteomics : MCP
|May 15, 2026
Summary
This study maps the heart’s nuclear proteome across chambers, revealing distinct regional signatures and a conserved core proteome. These findings offer a baseline for understanding nuclear regulation in heart health and disease.
Area of Science:
- Cardiovascular Biology
- Proteomics
- Molecular Cardiology
Background:
- Cardiac specialization relies on nuclear processes, but chamber-specific nuclear proteome regulation is largely unknown.
- Understanding the heart's nuclear proteome is crucial for deciphering cardiac function and disease.
Purpose of the Study:
- To construct a comprehensive, chamber-resolved nuclear proteome landscape of the healthy mouse heart.
- To identify regional proteome signatures and a conserved core nuclear proteome.
- To establish a baseline for studying nuclear remodeling in cardiac pathophysiology.
Main Methods:
- Quantitative mass spectrometry-based proteomics was used to analyze isolated nuclei from four major heart regions (left ventricle, right ventricle, left atrium, right atrium).
- Proteomic data was analyzed to identify regional signatures, chamber-specific features, and a conserved core nuclear proteome.
- Immunofluorescence was employed to verify the spatial localization of selected proteins.
Main Results:
- A comprehensive nuclear proteome landscape was constructed for left ventricle (2403 proteins), right ventricle (2242 proteins), left atrium (2368 proteins), and right atrium (1816 proteins).
- Distinct nuclear proteome signatures were discovered for ventricles (297 proteins) and atria (183 proteins), linked to metabolism, redox regulation, and transcriptional control.
- A conserved core nuclear proteome (230 proteins) was defined, enriched for transcription regulators, RNA processing, and chromatin organization factors, including 78 transcription factors/co-factors.
Conclusions:
- This study provides the first chamber-resolved view of the heart's nuclear proteome, revealing unique regional and conserved features.
- The identified nuclear proteomic signatures offer a framework for linking nuclear regulation to specific atrial and ventricular biology.
- This work serves as a foundational resource for future investigations into nuclear remodeling in cardiac diseases.
