Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Antenna Tuning in Photoredox Nitric Oxide Releasers for Potent Photovasodilation.

Journal of medicinal chemistry·2026

Same author

Substituent effects on photoresponsive nitric oxide release by <i>N</i>-nitrosoaniline derivatives.

Organic & biomolecular chemistry·2026

Same author

Revisiting X-linked agammaglobulinemia.

Journal of human immunity·2026

Same author

Posttransplant Chimerism Estimation Using Variant-Specific ddPCR in Inborn Errors of Immunity.

Transplantation and cellular therapy·2026

Same author

Development of silicon-fluorescein-based photolabile protecting groups with enhanced uncaging quantum yield.

RSC advances·2026

Same author

Rapid flow cytometric diagnosis of XIAP deficiency.

Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology·2026

Search research articles

Related Experiment Video

Updated: May 17, 2026

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

pH-Triggered, Time-Programmable Fluorescence within an Assembly-Free Single-Molecule System.

Rina Ueda¹, Kyoka Sakamoto², Madoka Nishimura²

¹Graduate School of Pharmacy, Aichi Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya 464-8650, Japan.

The Journal of Organic Chemistry

|May 15, 2026

Summary

This summary is machine-generated.

Researchers developed dynamic stimulus-responsive materials (DSRMs) with tunable optical changes for information security. These single-molecule scaffolds utilize pH-triggered reactions for novel encryption and display applications.

More Related Videos

Single-molecule Imaging of Gene Regulation In vivo Using Cotranslational Activation by Cleavage (CoTrAC)

Single-molecule Imaging of Gene Regulation In vivo Using Cotranslational Activation by Cleavage (CoTrAC)

Published on: March 15, 2013

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist

Published on: April 25, 2018

Related Experiment Videos

Last Updated: May 17, 2026

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

Single-molecule Imaging of Gene Regulation In vivo Using Cotranslational Activation by Cleavage (CoTrAC)

Single-molecule Imaging of Gene Regulation In vivo Using Cotranslational Activation by Cleavage (CoTrAC)

Published on: March 15, 2013

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist

Published on: April 25, 2018

Area of Science:

Materials Science
Organic Chemistry
Information Security

Background:

Dynamic stimulus-responsive materials (DSRMs) are crucial for advanced technologies.
Time-resolved optical changes in DSRMs offer potential for information security applications.

Purpose of the Study:

To design and construct single-molecule scaffold DSRMs with tunable time-resolved optical responses.
To develop a pH-responsive, fluorescence-based information encryption system using these DSRMs.

Main Methods:

Utilized pH-triggered, kinetically controlled spirocyclic ring-opening of leuco-triarylmethanes to rhodols.
Modulated electron-donating properties of amino substituents to tune response time scales.
Developed a fluorescence-based information encryption system exploiting spectral evolution.

Main Results:

Achieved distinct time-resolved spectral evolution in fluorescence intensity and absorbance.
Demonstrated precise tuning of material response time scales.
Successfully implemented a pH-responsive information encryption system.

Conclusions:

Single-molecule DSRMs based on leuco-triarylmethane to rhodol conversion offer tunable, time-resolved optical properties.
These materials provide a novel platform for developing advanced information encryption, storage, and display technologies.