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Updated: May 19, 2026

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
Published on: August 20, 2021
Complete reference genomes of Haemophilus ducreyi isolates ITG3105, CIP76118, and TMA3542, using Oxford Nanopore and
Daniel Golparian1, Daniel Schröder1, Magnus Unemo1,2
1World Health Organization Collaborating Centre for Gonorrhoea and other Sexually Transmitted Infections, Department of Laboratory Medicine, Microbiology, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
Abstract:
We report the complete reference genome sequences of three Haemophilus ducreyi isolates (ITG3105, CIP76118, and TMA3542), generated using Oxford Nanopore and Illumina sequencing, and hybrid assemblies. Each genome has a closed circular chromosome; ITG3105 and TMA3542 also have plasmids. These reference genomes support genomic, epidemiological, and antimicrobial resistance surveillance of H. ducreyi.

