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Updated: May 19, 2026

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
09:04

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

Genetic Engineering of Human Keratinocytes Using CRISPR/Cas9 Ribonucleoprotein Complexes or Modified Cas9-Encoding

Jaimy A Klijnhout1, Eline A W Senders1, Ellen H van den Bogaard1

  • 1Department of Dermatology, Research Institute for Medical Innovation, Radboud University Medical Center (Radboudumc), Nijmegen, The Netherlands.

Methods in Molecular Biology (Clifton, N.J.)
|May 18, 2026
PubMed
Abstract

Keywords:
CRISPR/Cas9Cationic vectorsElectroporationKeratinocyteLaboratory protocolLipofectamineN/TERT-1N/TERT-2GRibonucleoprotein

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