Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Multipotency and Niche of Bulge Stem Cell

Multipotency and Niche of Bulge Stem Cell

A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Effects of LATS1/2 kinases inhibition on lung epithelial cells in a regeneration model.

Molecular and cellular biochemistry·2026

Same author

The Evaluation of Fibrotic Alterations in Regenerated Murine and Human Xenografted Skin.

Methods in molecular biology (Clifton, N.J.)·2026

Same author

Characterization, Preconditioning, Safety, and Other Issues of MSC-Derived EVs and Secretome.

International journal of molecular sciences·2026

Same author

Why and How Are Infants with Hutchinson-Gilford Progeria Syndrome Born Without Severe Manifestations?

Medical sciences (Basel, Switzerland)·2025

Same author

Improvement of Treg Selectivity and Stability for Diabetes Mellitus Type 1 Treatment: Complex Approach for Perspective Technologies.

Cells·2025

Same author

Human Blastoid: A Next-Generation Model for Reproductive Medicine?

Biology·2025

Search research articles

Related Experiment Video

Updated: May 19, 2026

Generation and Culturing of Primary Human Keratinocytes from Adult Skin

Generation and Culturing of Primary Human Keratinocytes from Adult Skin

Published on: December 22, 2017

Generation of Hair Follicle Germs In Vitro Using Human Postnatal Skin Cells.

Ekaterina P Kalabusheva¹, Ekaterina A Vorotelyak^1,2

¹Koltzov Institute of Developmental Biology of Russian Academy of Sciences, Moscow, Russia.

Methods in Molecular Biology (Clifton, N.J.)

|May 18, 2026

Summary

This summary is machine-generated.

Researchers developed a new method to create hair follicle organoids from adult human skin cells. This breakthrough aids alopecia drug discovery and understanding hair regeneration.

Keywords:

Cell culture Dermal papilla Hair follicle Hair germ Keratinocytes Organoid

More Related Videos

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

Published on: December 14, 2015

Generation of Human Induced Pluripotent Stem Cell-derived Planar Hair-bearing Skin Organoids Using an Air-Liquid Interface Culture System

Generation of Human Induced Pluripotent Stem Cell-derived Planar Hair-bearing Skin Organoids Using an Air-Liquid Interface Culture System

Published on: October 17, 2025

Related Experiment Videos

Last Updated: May 19, 2026

Generation and Culturing of Primary Human Keratinocytes from Adult Skin

Generation and Culturing of Primary Human Keratinocytes from Adult Skin

Published on: December 22, 2017

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

Published on: December 14, 2015

Generation of Human Induced Pluripotent Stem Cell-derived Planar Hair-bearing Skin Organoids Using an Air-Liquid Interface Culture System

Generation of Human Induced Pluripotent Stem Cell-derived Planar Hair-bearing Skin Organoids Using an Air-Liquid Interface Culture System

Published on: October 17, 2025

Area of Science:

Regenerative Medicine
Dermatology
Stem Cell Biology

Background:

Organoid modeling offers a promising avenue for studying human hair follicle development and regeneration.
Current methods for in vitro hair follicle reconstruction face challenges in efficiency and scalability.
Understanding the molecular interplay between dermal papilla cells and keratinocytes is crucial for hair follicle morphogenesis.

Purpose of the Study:

To establish a highly efficient protocol for generating hair follicle germ-like organoids from adult human skin cells.
To investigate the self-organization potential of dermal papilla cells and keratinocytes in vitro.
To provide a model system for alopecia drug discovery and potential hair replacement therapies.

Main Methods:

Utilizing adult human skin-derived dermal papilla cells and epidermal keratinocytes.
Employing a hanging drop culture technique for self-organization.
Culturing isolated cells for two weeks to observe organoid formation.

Main Results:

Successful generation of hair follicle germ-like organoids exhibiting key features of early folliculogenesis.
Demonstrated self-organization of dermal papilla cells and keratinocytes into complex structures.
The protocol is efficient and applicable to single-donor adult human skin cells.

Conclusions:

The developed organoid model provides a robust platform for studying human hair follicle regeneration in vitro.
This approach facilitates the screening of potential alopecia therapeutics.
The organoids represent a significant step towards in vitro hair follicle replacement strategies.