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Updated: May 19, 2026
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Chemical-Induced Skin Carcinogenesis Model Using Dimethylbenz[a]Anthracene and 12-O-Tetradecanoyl Phorbol-13-Acetate (DMBA-TPA)
Published on: December 19, 2019
Method to Study Skin Cancer: Two-Stage Chemically Induced Carcinogenesis in Mouse Skin
Andrei N Mardaryev1, Iqra Fatima2, Andrey A Sharov3
1The Sablons Partnership, St Helier, Jersey, Channel Islands, France.
Abstract:
Two-stage chemical carcinogenesis method is widely used to elucidate genetic and molecular changes that lead to skin cancer development, as well as to test chemotherapeutic properties of novel drugs. This protocol allows researchers to reliably induce benign papilloma development and their conversion to squamous cell carcinoma in the skin of susceptible mouse strains in response to a single dose of carcinogen 2,4-dimethoxybenzaldehyde (DMBA) and repetitive applications of tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA).
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