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Updated: May 20, 2026

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

Protocol for total RNA sequencing analysis of extracellular RNA from biofluids

Anneleen Decock1, Kimberly Verniers1, Philippe Decruyenaere2

  • 1Department of Biomolecular Medicine, Ghent University, 9000 Ghent, Belgium; OncoRNALab, Cancer Research Institute Ghent (CRIG), 9000 Ghent, Belgium.

STAR Protocols
|May 18, 2026
PubMed
Abstract

Keywords:
RNA-seqSequence analysisSequencing

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RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...