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Related Experiment Video

Updated: May 20, 2026

An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment

An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment

Published on: December 3, 2020

Quercetin Alleviates Intestinal and Liver Dysfunction Induced by Hypoxia in a Microphysiological System.

Zhuan Liu^1,2, Lin Li^1,2, Tingting Yang^1,2

¹School of Biomedical Engineering, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230026, China.

ACS Biomaterials Science & Engineering

|May 18, 2026

Summary

Related Concept Videos

Hypoxia

Hypoxia

Hypoxia is a medical condition characterized by an inadequate oxygen supply to body tissues. It typically manifests as a bluish discoloration of the skin and mucosae, especially in fair-skinned individuals, when hemoglobin (Hb) saturation drops below 75%.
Types of Hypoxia
There are four primary types of hypoxia, each resulting from a different cause:
1. Anemic hypoxia: This type occurs due to insufficient oxygen delivery caused by a lack of red blood cells (RBCs) or RBCs with abnormal or...

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Pathological hypoxia harms the gut and liver. A new biomimetic gut-liver microphysiological system (GLMPS) models these effects, revealing quercetin

Area of Science:

Physiology
Toxicology
Biomedical Engineering

Background:

Pathological hypoxia, like high-altitude exposure, poses systemic health risks.
Hypoxia's specific impact on the intestine and liver is not fully understood due to model limitations.

Purpose of the Study:

To investigate hypoxia-induced injury mechanisms in the gut-liver axis.
To establish a biomimetic gut-liver microphysiological system (GLMPS) for studying these effects.

Main Methods:

Co-culturing human gut (Caco-2/HT29) and liver (HepG2) cell lines in a GLMPS.
Exposing cells to 1% O2 for 48 hours to induce hypoxia.
Utilizing transcriptomic analysis and assessing cellular responses.

Main Results:

Keywords:

gut−liver axis hypoxia microphysiological system (MPS)quercetin

Related Experiment Videos

Last Updated: May 20, 2026

An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment

An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment

Published on: December 3, 2020

Hypoxia increased intestinal barrier permeability, inflammation, and apoptosis in gut and liver cells.
Transcriptomics revealed shared endoplasmic reticulum stress and apoptosis pathways.
Quercetin treatment mitigated hypoxia-induced oxidative stress and apoptosis via autophagy enhancement.

Conclusions:

The GLMPS serves as a viable biomimetic in vitro platform for studying gut-liver axis pathologies.
This model facilitates the exploration of therapeutic targets for hypoxia-related diseases.