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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

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Related Experiment Video

Updated: May 20, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
06:34

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Published on: July 11, 2016

Challenges in Diagnosing Acanthamoebae Infections Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight

Ana Rosa Pellico1,2,3, Jörn Oliver Sass4, Patrick Leander Scheid5

  • 1Medical Parasitology Lab, Central Military Hospital Koblenz, Andernacher Str. 100, 56070, Koblenz, Germany. ana.pellico@gmx.de.

Acta Parasitologica
|May 19, 2026
PubMed
Summary
This summary is machine-generated.

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF MS) was evaluated for diagnosing Acanthamoebae infections. The method proved unreproducible, failing to establish a reliable diagnostic alternative for Acanthamoebiasis.

Keywords:
AcanthamoebaeDiagnosticsFree-living amoebaeMALDI-ToF MSMass spectrometryMicrobial identification

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Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
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Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa

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Last Updated: May 20, 2026

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Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
07:49

Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa

Published on: September 9, 2013

Area of Science:

  • Medical Parasitology
  • Microbiology
  • Analytical Chemistry

Background:

  • Acanthamoebae are protozoa causing severe human diseases like granulomatous amoebic encephalitis and Acanthamoeba-keratitis.
  • Rapid and reliable diagnostic methods are crucial for managing Acanthamoebiasis.
  • Previous studies on MALDI-ToF MS for Acanthamoeba identification yielded conflicting results.

Purpose of the Study:

  • To develop a time-saving and cost-effective method for identifying Acanthamoebae using MALDI-ToF MS.
  • To assess the reproducibility of MALDI-ToF MS based on characteristic protein profiles of Acanthamoebae.
  • To compare MALDI-ToF MS with real-time polymerase chain reaction for Acanthamoebiasis diagnostics.

Main Methods:

  • Xenically cultured Acanthamoeba strains were used for analysis.
  • Inactivation methods including UVC, heat, and centrifugal membrane filtration were tested to remove bacterial protein interference.
  • Protein profiles were analyzed using MALDI-ToF MS.

Main Results:

  • The mass spectra generated for Acanthamoebae identification were not reproducible.
  • The tested inactivation methods did not resolve the reproducibility issues.
  • MALDI-ToF MS characterization of Acanthamoebae was not successfully established in this study.

Conclusions:

  • The development of a diagnostic method for Acanthamoebae using MALDI-ToF MS was not achieved.
  • MALDI-ToF MS is not currently a suitable alternative to real-time polymerase chain reaction for diagnosing Acanthamoebiasis.
  • Further research is needed to explore alternative proteomic approaches for Acanthamoeba diagnostics.