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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Multispectral extended depth-of-field fluorescence microscopy with co-designed meta-optics and neural reconstruction.

Ipek Anil Atalay Appak1,2, Haobijam Johnson Singh1, Sanna Korpela3

  • 1Physics Unit, Faculty of Engineering and Natural Sciences, Tampere University, Tampere, 33720, Finland.

Light, Science & Applications
|May 19, 2026
PubMed
Summary
This summary is machine-generated.

MANTIS (Multispectral All-Depth meta-opTic Imaging System) extends microscope depth-of-field using a meta-optic and AI. This computational microscopy approach achieves sharp, in-focus images from thick specimens in a single shot.

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Published on: December 9, 2013

Area of Science:

  • Biomedical optics
  • Computational imaging
  • Microscopy

Background:

  • High numerical aperture microscopy offers subcellular resolution but suffers from a limited depth of field.
  • Thick specimens require axial scanning, and multispectral imaging exacerbates issues with chromatic aberrations and focus planes.
  • Existing methods struggle to maintain focus and registration across spectral channels in thick samples.

Purpose of the Study:

  • To develop a computational microscopy system for extended depth-of-field imaging without axial scanning.
  • To enable sharp, in-focus multispectral imaging of thick biological specimens.
  • To overcome the limitations of conventional microscopy in depth and spectral imaging.

Main Methods:

  • Co-designed a system combining a learned meta-optic with a physics-guided neural network.
  • Trained the neural network end-to-end to reconstruct images from depth and wavelength-dependent blurred sensor data.
  • Integrated the meta-optic and computational reconstruction for multispectral, extended depth-of-field imaging.

Main Results:

  • Achieved a 50 μm extended depth of field at NA 1.1, an 82-fold increase over conventional wide-field microscopy.
  • Demonstrated consistent performance across spectral channels with reduced defocus blur in thick specimens.
  • Validated on 50 μm thick 3D cultured MDCK II spheroids, maintaining contrast and lateral detail.

Conclusions:

  • MANTIS provides a significant advancement in imaging thick specimens with extended depth-of-field and multispectral capabilities.
  • The system overcomes key limitations of conventional microscopy, enabling clearer visualization of complex biological structures.
  • This computational approach offers a powerful tool for advanced fluorescence microscopy applications.