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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Related Experiment Video

Updated: May 21, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

A modular multi-color fluorescence microscope for simultaneous tracking of cellular activity and behavior.

Euphrasie Ramahefarivo^1,2, Leonard Böger^1,2,3, Takkasila Saichol¹

¹Max Planck Research Group Neural Information Flow, Max Planck Institute for Neurobiology of Behavior-Caesar, Bonn, Germany.

Nature Communications

|May 19, 2026

Summary

We developed an affordable, modular epifluorescence microscope from commercial parts for tracking moving biological structures. This accessible system enables imaging of neurons, muscles, and inter-species interactions in various model organisms.

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Last Updated: May 21, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

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Published on: June 30, 2018

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Area of Science:

Biophysics
Microscopy
Neuroscience

Background:

Advanced microscopy techniques are crucial for observing dynamic biological processes in vivo.
Existing systems can be expensive and complex, limiting accessibility for research and education.

Purpose of the Study:

To present a modular, affordable, and rapidly assembled epifluorescence tracking microscope.
To demonstrate its utility across diverse biological imaging applications, including neuroscience and developmental biology.

Main Methods:

Assembly of a modular microscope using commercially available components within 3 hours.
Application of ratiometric and dual-color fluorescence imaging techniques.
Utilizing genetically encoded indicators and fluorophores for enhanced signal and reduced photobleaching.

Main Results:

Successful calcium imaging of neurons in behaving Caenorhabditis elegans.
Analysis of muscle dynamics in Drosophila melanogaster larvae.
Visualization of predatory nematode-prey interactions using dual-color fluorescence.
Demonstration of brightfield imaging for tardigrade gait analysis.

Conclusions:

The presented microscope is a broadly accessible, cost-effective tool for live-animal imaging.
Its modularity and ease of use support diverse research applications and educational purposes.
The system facilitates advanced imaging of neural activity, muscle function, and ecological interactions.