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Updated: May 21, 2026

Unraveling the Unseen Players in the Ocean - A Field Guide to Water Chemistry and Marine Microbiology
Published on: November 5, 2014
Exploring the Ocean's Microbial World: Techniques and Protocols for Microbiome Research
Vijaya Raghavan Rangamaran1, T J Sushmitha2, Karthikeyan Kathan Tamilmani2
1Marine Biotechnology Group, National Institute of Ocean Technology (NIOT), Ministry of Earth Sciences (MoES), Government of India, Chennai, India. vijay.niot@gov.in.
Abstract:
Marine microbiomes play a crucial role in oceanic ecosystems, influencing biogeochemical cycles, climate regulation, and marine biodiversity. Accurate characterization of these microbial communities requires standardized protocols for sample collection, processing, sequencing and data analysis. This chapter provides a comprehensive guide to essential methodologies for marine microbiome research including field sampling strategies, DNA and RNA extraction techniques, high-throughput sequencing approaches (such as 16S rRNA amplicon sequencing and metagenomics) and bioinformatics pipelines for data interpretation. Additionally, we discuss quality control measures, best practices for reproducibility, and challenges associated with marine microbiome profiling. By adopting standardized methodologies, researchers can generate reliable, comparable datasets that enhance our understanding of marine microbial ecology and its broader environmental implications.
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