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Unraveling the Unseen Players in the Ocean - A Field Guide to Water Chemistry and Marine Microbiology

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Published on: November 5, 2014

Exploring the Ocean's Microbial World: Techniques and Protocols for Microbiome Research.

Vijaya Raghavan Rangamaran¹, T J Sushmitha², Karthikeyan Kathan Tamilmani²

¹Marine Biotechnology Group, National Institute of Ocean Technology (NIOT), Ministry of Earth Sciences (MoES), Government of India, Chennai, India. vijay.niot@gov.in.

Methods in Molecular Biology (Clifton, N.J.)

|May 19, 2026

Summary

Standardized methods are essential for accurately characterizing marine microbiomes. This guide details marine microbiome research protocols, from sampling to data analysis, ensuring reliable ecological insights.

Keywords:

Bioinformatics analysis Marine microbiome Sampling protocols Whole metagenome shotgun sequencing

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Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

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Published on: March 19, 2018

Area of Science:

Marine microbiology
Oceanography
Ecology

Background:

Marine microbiomes are vital to oceanic ecosystems, influencing biogeochemical cycles, climate, and biodiversity.
Accurate characterization of these communities necessitates standardized research protocols.

Purpose of the Study:

To provide a comprehensive guide to essential methodologies for marine microbiome research.
To outline best practices for sample collection, processing, sequencing, and data analysis.

Main Methods:

Field sampling strategies
DNA and RNA extraction techniques
High-throughput sequencing (16S rRNA amplicon sequencing, metagenomics)
Bioinformatics pipelines for data interpretation

Main Results:

The chapter details standardized protocols for marine microbiome research.
It covers quality control, reproducibility, and challenges in profiling.

Conclusions:

Adopting standardized methodologies generates reliable, comparable datasets.
This enhances understanding of marine microbial ecology and its environmental implications.