A disposable molecularly imprinted electrochemical sensing electrode for sub-picomolar histamine detection based on a
Suntisak Khumngern1, Phennapa Khanaaekwichaporn2, Apon Numnuam3
1Center of Excellence for Trace Analysis and Biosensor, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.
Abstract:
A disposable and selective electrochemical sensor based on Prussian blue-embedded molecularly imprinted polypyrrole (MIP@PB) and an AuNPs@MXene nanocomposite was developed for the detection of histamine (HA) in fish samples. The MIP@PB served as a selective polymer for HA, while the AuNPs@MXene as a support material provided a high surface area, excellent conductivity, and efficient electron transfer, thereby enhancing sensor sensitivity. Molecular docking studies indicated that the strong MIP - HA affinity originated from hydrogen bonding, π-π stacking, and hydrophobic interactions. Under optimized conditions, the sensor detected HA via a decrease in PB oxidation current using differential pulse voltammetry. It exhibited a linear detection range from 1 × 10-5 to 1 × 105 nM and a detection limit of 7.5 × 10-6 nM. The sensor also showed high selectivity in real fish samples and produced results consistent with those obtained by ELISA. This work highlights a promising platform for portable HA detection, offering potential for food safety monitoring.
