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Related Concept Videos

Neurulation01:30

Neurulation

Neurulation is the embryological process which forms the precursors of the central nervous system and occurs after gastrulation has established the three primary cell layers of the embryo: ectoderm, mesoderm, and endoderm. In humans, the majority of this system is formed via primary neurulation, in which the central portion of the ectoderm—originally appearing as a flat sheet of cells—folds upwards and inwards, sealing off to form a hollow neural tube. As development proceeds, the anterior...

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Cranial Neural Crest Cells Three-Dimensional In Vitro Differentiation Protocol for Multiplexed Assay
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Embryonic Gut Enteric Neural Crest Cells Derived Neurospheres.

Dorothy Lee1, Naho Fujiwara1,2, Agostino Pierro3

  • 1Translational Medicine, Division of General and Thoracic Surgery, The Hospital for Sick Children, Toronto, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|May 20, 2026
PubMed
Summary

Researchers developed a method to isolate enteric neural crest cells (ENCCs) from mouse embryos. This technique uses neural selective media to create ENCC-derived neurospheres for studying gut nervous system development.

Keywords:
Embryonic gutEnteric nervous system (ENS)Enteric neural crest cells (ENCCs)Neural selective mediaNeurospheres

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Area of Science:

  • Developmental biology
  • Neuroscience
  • Stem cell biology

Background:

  • Enteric neural crest cells (ENCCs) are crucial for forming the enteric nervous system (ENS).
  • Understanding ENCC development is key to addressing congenital gut disorders.
  • Existing methods for ENCC isolation are challenging due to low cell numbers.

Purpose of the Study:

  • To establish an efficient method for generating ENCC-derived neurospheres.
  • To facilitate in vitro studies of ENS development and molecular pathways.
  • To provide a model for investigating ENS morphogenesis.

Main Methods:

  • Isolation of ENCCs from mouse embryonic gut at embryonic day 13.5.
  • Culture of ENCCs in neural selective media.
  • Formation of ENCC-derived neurospheres.

Main Results:

  • Successful generation of ENCC-derived neurospheres.
  • Enrichment of ENCC population through neural selective media.
  • Establishment of a reproducible method for ENCC culture.

Conclusions:

  • The described method effectively generates ENCC-derived neurospheres.
  • This approach supports further research into ENS development and related diseases.
  • The neurosphere model offers a valuable tool for studying ENCC behavior in vitro.