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Gastrulation

Gastrulation

Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata will form...

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Related Experiment Video

Updated: May 22, 2026

A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors

A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors

Published on: September 18, 2013

Embryonic Gut Explant Model for Enteric Neural Crest Cells Migration.

Dorothy Lee¹, Agostino Pierro²

¹Translational Medicine, Division of General and Thoracic Surgery, The Hospital for Sick Children, Toronto, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)

|May 20, 2026

Summary

This summary is machine-generated.

This study introduces a new mouse model to track enteric neural crest cells (ENCCs) during gut development. This model aids in understanding enteric nervous system (ENS) formation and related birth defects.

Keywords:

Cell migration Enteric nervous system (ENS)Enteric neural crest cells (ENCCs)Explant Live imaging

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Area of Science:

Developmental biology
Neuroscience
Genetics

Background:

Enteric neural crest cells (ENCCs) migrate to form the enteric nervous system (ENS).
Understanding ENCC migration is crucial for addressing ENS developmental defects like Hirschsprung disease.
Current study models have limitations for live tracing of migrating ENCCs.

Purpose of the Study:

To develop and validate a novel mouse embryonic gut explant model.
To enable live-cell imaging of ENCC migration within the developing intestine.
To facilitate research into ENS development and pathogenesis of related disorders.

Main Methods:

Utilized a Sox10-Venus transgenic mouse model.
Established a mouse embryonic gut explant culture system.
Employed live-cell imaging techniques to track fluorescently labeled ENCCs.

Main Results:

Successfully labeled migrating ENCCs using Venus fluorescence.
Demonstrated the feasibility of live imaging of ENCCs within cultured gut explants.
Provided a robust model for studying ENCC migration dynamics.

Conclusions:

The described mouse embryonic gut explant model is effective for studying ENCC migration.
This model offers a valuable tool for investigating ENS development and diseases.
It overcomes limitations of previous models for live cell tracing.