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Updated: May 22, 2026
08:07
Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
Published on: April 12, 2019
OTX1 promoting osteosarcoma malignancy by activating PTGS2 transcription
Guangjian Bai1, Zhao Li1, Zhongfei Huang2
1University of Shanghai for Science and Technology, School of Health Science and Engineering Shanghai, China.
American Journal of Cancer Research
|May 21, 2026
Summary
Orthodenticle homeobox 1 (OTX1) drives osteosarcoma progression by increasing Prostaglandin-endoperoxide synthase 2 (PTGS2) expression. Targeting this OTX1/PTGS2 pathway offers a potential therapeutic strategy for aggressive bone cancer.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- Osteosarcoma (OS) is an aggressive bone cancer with poor therapeutic outcomes, especially for metastatic or recurrent cases.
- The role of transcription factor Orthodenticle homeobox 1 (OTX1) in OS pathogenesis is not well understood.
- Limited therapeutic options exist for advanced osteosarcoma.
Purpose of the Study:
- To investigate the expression, function, and mechanism of OTX1 in osteosarcoma.
- To explore the potential of targeting the OTX1/PTGS2 axis for OS treatment.
Main Methods:
- Examined OTX1 expression in OS tissues and cell lines.
- Performed functional assays to assess OTX1's impact on OS cell proliferation, migration, and invasion.
- Conducted mechanistic studies to identify OTX1's downstream targets, including Prostaglandin-endoperoxide synthase 2 (PTGS2).
- Utilized PTGS2 inhibitor celecoxib in in vitro and in vivo OS models.
Main Results:
- OTX1 was aberrantly upregulated in OS tissues and correlated with poor patient prognosis.
- OTX1 overexpression promoted OS cell proliferation, migration, and invasion.
- OTX1 transcriptionally activated PTGS2, which mediated anti-apoptotic and pro-invasive effects.
- Celecoxib treatment reversed OTX1-induced tumor-promoting effects and showed tumor-suppressive activity in vivo.
Conclusions:
- OTX1 promotes osteosarcoma progression by upregulating PTGS2, impacting apoptosis and invasion.
- The OTX1/PTGS2 signaling axis represents a potential therapeutic target for osteosarcoma, particularly in high-risk patients.
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