Related Experiment Video
Updated: May 22, 2026

Isolation, Culture, and Adipogenic Induction of Stromal Vascular Fraction-derived Preadipocytes from Mouse Periaortic Adipose Tissue
Published on: July 21, 2023
PPARγ-dependent transcriptional regulation of Vaspin expression in adipocytes
Daisuke Aibara1, Kimihiko Matsusue1
1Faculty of Pharmaceutical Sciences, Fukuoka University, Jonan-ku, Fukuoka, Japan.
Abstract:
Vaspin is an adipokine involved in metabolic regulation; however, the mechanisms governing its transcription in obesity remain unclear. Here, we investigated the role of peroxisome proliferator-activated receptor γ (PPARγ) in regulating Vaspin expression in white adipose tissue (WAT). Vaspin expression was markedly reduced in the WAT of obese ob/ob mice and was restored following rosiglitazone treatment. Reporter assays revealed that ligand-activated PPARγ promotes Vaspin transcription through a weak but functional PPAR response element (PPRE). Promoter deletion analysis further showed that this PPRE contributes to transcriptional activation, whereas additional promoter regions containing C/EBP-responsive elements sustain residual transcriptional activity. These findings suggest that Vaspin expression is regulated, at least partly, through a noncanonical PPARγ-dependent mechanism involving cooperative transcriptional regulation. Our findings provide insight into adipokine regulation in obesity and highlight gene-specific differences in PPARγ-mediated transcription.
More Related Videos
Related Concept Videos
Cell Specific Gene Expression
Regulation of Angiogenesis and Blood Supply
Master Transcription Regulators
Regulation of Nuclear Protein Sorting
GPCRs Regulate Adenylyl Cylase Activity
Two...

