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HIV-1 virome profiling using HIV-PULSE to guide therapeutic and curative interventions
Sofie De Braekeleer1, Laurens Lambrechts1, Liesbet Termote1
1HIV Cure Research Center, Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium.
The HIV-PULSE assay characterizes the HIV-1 reservoir in individuals on therapy. This comprehensive profiling advances personalized treatment strategies and the pursuit of an HIV-1 cure.
Area of Science:
- Virology
- Immunology
- Genomics
Background:
- The persistent Human Immunodeficiency Virus type 1 (HIV-1) reservoir is a significant obstacle to achieving a cure.
- Understanding the reservoir's composition is crucial for developing effective targeted interventions.
Purpose of the Study:
- To characterize the viral reservoir in individuals receiving suppressive antiretroviral therapy using the HIV Proviral Unique molecular identifier-mediated Long-read Sequencing (HIV-PULSE) assay.
- To enable comprehensive in silico analyses of near full-length proviral sequences.
Main Methods:
- Application of the HIV-PULSE assay to 90 individuals on suppressive antiretroviral therapy.
- Generation of near full-length proviral sequences for detailed genomic analysis.
Main Results:
- Construction of participant-specific HIV virome profiles.
- Integration of assessments including proviral intactness, tropism, genetic diversity, archived drug resistance mutations, and susceptibility to immune-based therapies (e.g., broadly neutralizing antibodies, IMC-M113V).
Conclusions:
- HIV-PULSE serves as a comprehensive platform for individualized reservoir profiling.
- The findings contribute to advancing future treatment perspectives and strategies aimed at an HIV-1 cure.
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