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Updated: May 23, 2026

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry
Published on: June 26, 2019
HIV-1 virome profiling using HIV-PULSE to guide therapeutic and curative interventions
Sofie De Braekeleer1, Laurens Lambrechts1, Liesbet Termote1
1HIV Cure Research Center, Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium.
Background:
The persistent HIV-1 reservoir remains a major barrier to curing HIV-1, underscoring the need for a detailed understanding of its composition to inform targeted interventions.
Methods:
Here, we apply the HIV Proviral Unique molecular identifier-mediated Long-read Sequencing (HIV-PULSE) assay to characterise the viral reservoir in 90 individuals on suppressive antiretroviral therapy. By generating near full-length proviral sequences, HIV-PULSE enables multiple layers of in silico analyses across the HIV-1 genome.
Findings:
These data support the construction of participant-specific HIV virome profiles, integrating assessments of proviral intactness, tropism, genetic diversity, archived drug resistance mutations and susceptibility to selected immune-based therapies (broadly neutralising antibodies and HIV-specific immune-mobilising monoclonal T cell receptor therapy IMC-M113V).
Interpretation:
Our findings demonstrate that HIV-PULSE provides a comprehensive platform for individualised reservoir profiling, advancing future treatment perspectives and efforts towards an HIV-1 cure.
Funding:
This research is part of the 2000HIV study, supported by ViiV Healthcare.
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