Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Nitric Oxide Signaling Pathway01:28

Nitric Oxide Signaling Pathway

Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure to...
What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

S-nitrosylation of protein kinase A is required for its activation by GPCRs.

bioRxiv : the preprint server for biology·2026
Same author

Computational Quantification of Peristalsis in Preclinical Mouse Models Using Smartphone Videography.

Neurogastroenterology and motility·2026
Same author

Inherent Specificity and Variation Sensitivity as Quantitative Metrics for RBP Binding.

bioRxiv : the preprint server for biology·2026
Same author

Predictors for T cell receptor excision circles in infants without severe combined immunodeficiency or thymic aplasia/hypoplasia.

Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology·2026
Same author

Higher diabetes genetic load in proliferative diabetic retinopathy in South India: The South Indian GeNetics of DiAbeTic Retinopathy (SIGNATR) study.

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie·2026
Same author

The protein denitrosylase SCoR2 regulates lipogenesis and fat storage.

Science signaling·2025

Related Experiment Video

Updated: May 23, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Nitric oxide drives proteomic diversity through alternative splicing.

Joseph C Schindler1, Puneet Seth2, Alfred Hausladen3

  • 1Institute for Transformative Molecular Medicine, Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA; Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA.

Molecular Cell
|May 21, 2026
PubMed
Summary
This summary is machine-generated.

Nitric oxide (NO) modifies RNA-binding proteins (RBPs) through S-nitrosylation, impacting gene expression and alternative splicing. This redox regulation is crucial in diseases like Alzheimer's.

Keywords:
CLIP-seqPTBP1RNA-binding proteinsS-nitrosylationalternative splicinggasotransmitternitric oxideredox signaling

More Related Videos

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
08:32

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Published on: March 16, 2017

Related Experiment Videos

Last Updated: May 23, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
08:32

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Published on: March 16, 2017

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Nitric oxide (NO) mediates redox signaling through S-nitrosylation (SNO-modification), affecting a significant portion of the proteome.
  • RNA-binding proteins (RBPs) are identified as a major class of S-nitrosylation targets, particularly spliceosomal factors.

Purpose of the Study:

  • To investigate the role of NO-mediated S-nitrosylation in regulating alternative splicing (AS) and gene expression.
  • To characterize the impact of S-nitrosylation on the function of PTBP1, a key AS regulator.

Main Methods:

  • Proteomic analysis to identify S-nitrosylation targets.
  • Biochemical assays to assess PTBP1 S-nitrosylation and its effects on protein function.
  • Analysis of gene expression and alternative splicing changes in response to PTBP1 S-nitrosylation.
  • Comparative analysis of SNO-RBP conservation.

Main Results:

  • RBPs, especially spliceosomal factors, are significantly enriched among S-nitrosylation targets.
  • S-nitrosylation of PTBP1 alters RNA-binding domain conformation, RNA recognition, protein interactions, and trafficking, leading to substantial gene expression shifts.
  • Reduced SNO-PTBP1 levels are observed in Alzheimer's disease brains and correlate with clinical outcomes.
  • S-nitrosylated RBPs (SNO-RBPs) show cross-lineage conservation.

Conclusions:

  • NO-mediated S-nitrosylation of RBPs is a key mechanism for regulating alternative splicing and gene expression.
  • Dysregulation of SNO-PTBP1 is implicated in neurodegenerative diseases like Alzheimer's.
  • SNO-RBPs provide a framework for understanding redox regulation of the transcriptome and proteome in health and disease.