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Related Experiment Video

Updated: May 23, 2026

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
10:34

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Published on: July 22, 2016

Using an RNA binding protein to detect mRNA on lipid nanoparticles.

Meagan McMahon1, Hareth Al-Wassiti2, Kirsten Vandenberg3

  • 1CSL Seqirus Ltd, Parkville, Australia. Meagan.mcmahon@seqirus.com.

Scientific Reports
|May 21, 2026
PubMed
Summary
This summary is machine-generated.

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Researchers developed a new assay to measure how well lipid nanoparticles (LNPs) encapsulate nucleic acids, crucial for effective gene therapy and vaccine delivery.

Area of Science:

  • Biotechnology
  • Nanomedicine
  • Molecular Biology

Background:

  • Lipid nanoparticles (LNPs) are key delivery vehicles for nucleic acids in vaccines and gene therapy.
  • Efficient delivery depends on complete encapsulation of nucleic acids within the LNP.
  • Quantifying surface-exposed vs. encapsulated nucleic acids is vital for LNP optimization.

Purpose of the Study:

  • To develop and validate an encapsulation assay for assessing RNA within LNPs.
  • To determine the encapsulation efficiency of standard RNA LNP formulations.
  • To provide a tool for optimizing LNP-based nucleic acid delivery systems.

Main Methods:

  • Utilized a viral RNA binding protein and its specific monoclonal antibody.
  • Employed a magnetic bead-based system for separation and quantification.

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A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA

Published on: December 2, 2009

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
10:52

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Published on: September 28, 2017

Related Experiment Videos

Last Updated: May 23, 2026

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
10:34

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Published on: July 22, 2016

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA

Published on: December 2, 2009

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
10:52

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Published on: September 28, 2017

  • Quantified encapsulated versus surface-exposed mRNA within LNPs.

    • Main Results:

    • Successfully developed an assay to differentiate surface-exposed from encapsulated RNA.
    • Demonstrated the ability to determine RNA encapsulation efficiency in LNPs.
    • The assay allows for manipulation of components to optimize encapsulation.

    Conclusions:

    • The developed assay is effective for evaluating RNA encapsulation in LNPs.
    • This method can aid in optimizing LNP formulations for enhanced nucleic acid delivery.
    • The assay has broad applications in advancing LNP technology for therapeutics and vaccines.