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Related Experiment Video

Updated: May 24, 2026

Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems
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A Rapid Binder Discovery Workflow for Engineering Mini-Protein Degraders.

Jingzhou Yang1, Matthew J Styles1, Kanokpol Aphicho1

  • 1Department of Chemistry, University of Chicago, Chicago, Illinois 60637, United States.

Journal of the American Chemical Society
|May 22, 2026
PubMed
Summary

This study introduces a rapid Phage-Assisted Non-Continuous Selection for Binders (PANCS-Binders) workflow for discovering cancer-related molecular binders. The technology quickly yielded verified binders and enabled the development of a novel cancer degrader.

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Last Updated: May 24, 2026

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Identifying the Binding Proteins of Small Ligands with the Differential Radial Capillary Action of Ligand Assay (DRaCALA)
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Identifying the Binding Proteins of Small Ligands with the Differential Radial Capillary Action of Ligand Assay (DRaCALA)

Published on: March 19, 2021

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Cancer Research

Background:

  • Developing selective binding molecules is a key challenge in biological research and biotechnology.
  • Existing methods for de novo binder discovery can be time-consuming.

Purpose of the Study:

  • To present and validate a rapid workflow for de novo binder discovery using Phage-Assisted Non-Continuous Selection for Binders (PANCS-Binders) technology.
  • To demonstrate the utility of this workflow for identifying binders against cancer-related targets.
  • To showcase the application of discovered binders in chemical biology and biotechnology.

Main Methods:

  • Utilized the PANCS-Binders technology for rapid de novo binder discovery.
  • Targeted three cancer-related proteins: NSD3, NMNAT2, and CSF1R.
  • Incorporated a discovered NSD3 binder into an engineered E3 ligase (RNF8) to create a degrader.

Main Results:

  • Successfully identified sequence- and function-verified binders for all three targets within 26 days.
  • Achieved nano- to micromolar affinities for the discovered binders.
  • Developed a potent NSD3 degrader that depleted endogenous NSD3 and inhibited colorectal cancer cell proliferation.
  • Uncovered novel NSD3 dependencies in ovarian cancer cell lines using the developed degrader.

Conclusions:

  • The PANCS-Binders workflow provides a robust and accelerated method for binder discovery.
  • Discovered binders can be effectively utilized to create molecular degraders for chemical biology research.
  • This approach facilitates the identification of new therapeutic vulnerabilities and accelerates biotechnology development.