Related Experiment Video
Updated: May 24, 2026
12:57
Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
Published on: December 21, 2017
In vivo single-cell ribosome profiling reveals cell-type-specific translational programs during aging
Clara Duré1, Umesh Ghoshdastider2, Ramona Weber2
1Institute for Regenerative Medicine (IREM), University of Zurich, Wagistrasse 12, 8952 Schlieren, Zurich, Switzerland; Life Science Zurich Graduate School, Molecular Life Science Program, University of Zurich, ETH Zurich, 8057 Zurich, Switzerland.
Molecular Cell
|May 22, 2026
Summary
Aging alters protein synthesis in skin stem cells. This study introduces a new method to map these changes, revealing specific shifts in aged epidermal stem cells that impact their function.
Area of Science:
- Molecular Biology
- Cell Biology
- Aging Research
Background:
- Somatic stem cells exhibit low protein synthesis, linked to stemness.
- The impact of aging on stem cell translational landscapes is largely unknown.
Purpose of the Study:
- To develop and apply an in vivo single-cell ribosome profiling strategy.
- To investigate the tissue-wide translational landscapes of the epidermis during aging.
- To identify cell-type-specific translational reprogramming in aged epidermal stem cells.
Main Methods:
- Developed an in vivo single-cell ribosome profiling strategy.
- Utilized ribosomal elongation inhibition and RNase I for enhanced data quality.
- Analyzed translational landscapes across major epidermal cell types.
Main Results:
- Documented in vivo translational landscapes of epidermal cells.
- Identified cell-type-specific translational efficiencies.
- Discovered significant translational reprogramming of AP-1 subunits in aged epidermal stem cells.
Conclusions:
- In vivo single-cell ribosome profiling is a powerful tool for mapping cell-type-specific translational programs.
- Aging induces specific translational reprogramming in epidermal stem cells.
- The developed strategy offers a scalable approach for tissue-wide translational analysis.
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