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Updated: May 24, 2026

Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications

Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications

Published on: April 13, 2022

Engineering biocompatible chitosan hydrogels with polyether-based crosslinkers for biomedical applications.

Nikolaos Politakos¹, Carlos A Busatto², Maria Soledad Orellano³

¹POLYMAT, Applied Chemistry Department, University of the Basque Country, UPV/EHU, Tolosa Avenue 72, 20018 Donostia-San Sebastián, Spain; Institute of Chemical Biology, National Hellenic Research Foundation, Leof. Vasileos Konstantinou 48, Athens, 11635, Greece.

International Journal of Biological Macromolecules

|May 22, 2026

Summary

Researchers developed versatile chitosan hydrogels using click chemistry for biomedical uses. Different crosslinkers allowed tuning of properties for applications like tissue scaffolds and drug delivery, with all materials proving non-cytotoxic.

Keywords:

Chitosan Dendritic polyglycerol Drug delivery HGs Nanogels PEG Tissue engineering

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Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture

Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture

Published on: September 29, 2017

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Last Updated: May 24, 2026

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Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture

Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture

Published on: September 29, 2017

Area of Science:

Biomaterials Science
Polymer Chemistry
Regenerative Medicine

Background:

Chitosan is a biocompatible polymer crucial for biomedical applications.
Tailoring chitosan hydrogels' properties is key for specific therapeutic needs.
Developing advanced biomaterials requires precise control over hydrogel characteristics.

Purpose of the Study:

Synthesize and characterize chitosan-based hydrogels (HGs) with tunable properties.
Investigate the impact of various crosslinkers (PEG, dPG, NGs) on HG performance.
Evaluate HGs for potential applications in regenerative medicine and drug delivery.

Main Methods:

Chitosan functionalization with thiol groups.
Thiol-ene click chemistry for hydrogel synthesis using acrylated crosslinkers.
Comprehensive characterization: NMR, SEM, swelling studies, rheology, and in vitro cytocompatibility assays.

Main Results:

Hydrogel properties (morphology, swelling, mechanical strength) varied with crosslinker type and concentration.
Linear PEG crosslinkers yielded HGs with high water absorption and storage moduli.
dPG and nanogel crosslinkers offered broader mechanical tunability and intermediate swelling, respectively.
All HG formulations demonstrated excellent cytocompatibility with human fibroblasts and keratinocytes.

Conclusions:

Chitosan hydrogels synthesized via thiol-ene click chemistry offer tunable properties for diverse biomedical applications.
The choice of crosslinker significantly influences hydrogel morphology, swelling, and mechanical behavior.
These versatile hydrogels show promise as platforms for regenerative medicine and controlled drug delivery systems.