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Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion
Published on: May 10, 2020
A triple-functional sandwich-like nanofibrous matrix for accelerated gingival augmentation
Lizhi Zeng1, Shanghui Huang2, Tim Forouzanfar2
1Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Key Laboratory of Regenerative Medicine of Ministry of Education, Guangdong Provincial Engineering and Technological Research Centre for Drug Carrier Development, Department of Biomedical Engineering, Jinan University, Guangzhou 510632, China.
A novel triple-functional nanofiber matrix effectively repairs keratinized gingival defects by mimicking natural gingiva's mechanical properties. This advanced biomaterial promotes tissue regeneration, offering a superior solution for oral and maxillofacial surgery.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Oral and Maxillofacial Surgery
Background:
- Keratinized gingival defect repair and soft tissue augmentation are critical in oral surgery.
- Current treatments are limited by poor antimicrobial properties, slow regeneration, and inflammation control.
Purpose of the Study:
- To design and validate a novel triple-functional nanofiber matrix for gingival defect repair.
- To assess its mechanical properties, antibacterial activity, and regenerative potential in vivo.
Main Methods:
- Fabrication of a "sandwich" structure nanofiber matrix using electrospinning.
- Inner layer: polycaprolactone (PCL) with zinc oxide nanoparticles (ZnO NPs).
- Outer layer: PCL blended with collagen (rhCol Ⅰ), ZnO NPs, and Histatin-1 (Hst-1).
Main Results:
- The matrix demonstrated Young's modulus similar to natural gingiva.
- It exhibited excellent antibacterial activity, promoting collagen regeneration and angiogenesis.
- In Beagle dogs, it achieved a soft tissue volume gain of 153 mm³ compared to Geistlich Mucograft® (63 mm³) and autologous grafting (92 mm³).
Conclusions:
- A multifunctional nanofiber scaffold was successfully developed.
- The scaffold offers mechanical strength, antibacterial properties, and promotes tissue regeneration.
- It significantly surpasses existing therapies in repairing keratinized gingival defects.
