MKK7 is involved in isoleucine-enhanced immunity in plants
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Updated: May 25, 2026

Identification of Post-translational Modifications of Plant Protein Complexes
Published on: February 22, 2014
Jiahui Liang1, Zhanhao Huang1, You Li1
1School of Life Sciences, State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, Sun Yat-sen University, NO. 135, Xingangxi Road, Guangzhou 510275, People's Republic of China.
Abstract:
Amino acids participate in the interaction between plants and microbes, regulating plant resistance to pathogens. Here, we demonstrate that the application of exogenous isoleucine (Ile) enhances defense against Botrytis cinerea in some Solanaceae plants and Arabidopsis. Interestingly, exogenous Ile application can enhance immune responses such as reactive oxygen species (ROS) burst and mitogen-activated protein kinase (MAPK) activation triggered by fungal and bacterial pathogen-associated molecular patterns (PAMPs) in Arabidopsis. Moreover, SERINE/THREONINE/TYROSINEKINASE 8 (STY8), STY17, and STY46 homologs and MAP kinase kinase 7 (MKK7) are involved in the Ile-enhanced MAPK activation triggered by fungal chitin and bacterial flagellin 22 (flg22) in Arabidopsis. Accordingly, MKK7 contributes to the Ile-enhanced plant defense to B. cinerea and Pseudomonas syringae pv tomato (Pto) DC3000 in Arabidopsis. Our results indicate a role for MKK7 in the precise modulation of plant immunity, as well as the potential of Ile as a plant immune inducer through the MKK7 pathway.
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