Location and organization of a complex, integrated transgenic array in a Caenorhabditis elegans strain carrying...

Hairy/Enhancer of Split (HES) proteins are critical for animal development and for the regulation of human diseases. The Caenorhabditis elegans genome encodes six HES orthologs, including HLH-29 . We used Nanopore sequencing on the MinION platform to define the location and organization of an integrated transgenic array expressing green fluorescent protein driven by the hlh-29 promoter. The array, ardIS501 , is at least 188.5 kb long, is inserted into Chromosome III of Bristol N2 , and contains at least 26 copies of hlh-29 p::gfp and 11 copies of rol-6 ( su1006 ) . The coding sequences Y46E12A.2 .1 and Y46E12A.5 .1 are deleted in this strain, with no observable phenotypes.