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Updated: May 26, 2026

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
Published on: April 11, 2014
Plasma membrane mediated GLUT10 mitochondrial targeting regulates intracellular ascorbic acid homeostasis
Anu Chirackal Jose1,2,3, Yu-Wei Syu1, Hao-Wen Lai1,4
1Institute of Cellular and Organismic Biology, Academia Sinica, Taipei 11529, Taiwan.
Cellular stress triggers a novel pathway where glucose transporter 10 (GLUT10) moves from the ER to the plasma membrane, enhancing ascorbic acid (AA) uptake and maintaining homeostasis.
Area of Science:
- Cell Biology
- Biochemistry
- Molecular Biology
Background:
- Intracellular ascorbic acid (AA) regulation is crucial for connective tissue health.
- Mechanisms controlling AA homeostasis during cellular stress are not fully understood.
Purpose of the Study:
- To identify the intracellular trafficking mechanisms of glucose transporter 10 (GLUT10) under oxidative stress.
- To elucidate the role of GLUT10 trafficking in maintaining ascorbic acid homeostasis.
Main Methods:
- Live-cell imaging to track GLUT10 movement.
- Biochemical assays to assess AA uptake and homeostasis.
- Investigating GLUT10 localization at the plasma membrane and endosomes.
Main Results:
- Oxidative stress induces GLUT10 trafficking from the ER to the plasma membrane (PM).
- Increased PM localization of GLUT10 enhances dehydroascorbic acid (DHA) uptake, supporting intracellular AA levels.
- Disruption of this GLUT10 trafficking pathway impairs AA homeostasis.
Conclusions:
- A novel endomembrane-to-mitochondria pathway involving GLUT10 trafficking maintains AA homeostasis.
- This mechanism supports cellular adaptation to oxidative stress.
- Uncovers previously unrecognized GLUT10 localization and function in endomembrane-mitochondria communication.
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