Related Experiment Video
Updated: May 26, 2026
09:51
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
Published on: May 25, 2018
PrEditR: A protein-centric platform for CRISPR-mediated base editor sgRNA design.
Felipe Vasquez-Castro1,2, Leonardo D Sanchez Solis1, Samuel A Myers1,3,4,5
1Laboratory for Immunochemical Circuits, La Jolla Institute for Immunology, La Jolla, CA 92037, United States of America.
Biorxiv : the Preprint Server for Biology
|May 25, 2026
Summary
PrEditR is a new tool for designing CRISPR base editor screens to study protein function. It enables high-throughput analysis of post-translational modification sites by focusing on protein sequences.
Area of Science:
- Molecular Biology
- Proteomics
- Genetics
Background:
- Post-translational modifications (PTMs) are crucial for protein function, but the roles of many modification sites are unknown.
- CRISPR-based screens with base editors are effective for studying PTMs at scale.
- Current single-guide RNA (sgRNA) design tools are DNA-focused and do not integrate well with proteomics data.
Purpose of the Study:
- To develop a protein-centric tool for designing sgRNA for base editor screens.
- To facilitate high-throughput investigation of PTM function.
- To bridge the gap between genetic screening and proteomics.
Main Methods:
- Introduction of PrEditR, an open-source, protein-centric software.
- Design of custom base editor screens targeting specific amino acid residues.
- Generation of protospacer sequences for endogenous gene targeting.
Main Results:
- PrEditR enables high-throughput sgRNA design for base editor screens.
- The tool allows users to specify amino acid residues for targeted mutations.
- Facilitates the installation of missense mutations via base editors.
Conclusions:
- PrEditR offers a powerful solution for dissecting PTM function.
- The protein-centric approach enhances the study of protein modifications.
- PrEditR is readily available for researchers via GitHub and Docker Hub.
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