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Related Concept Videos

Stem Cell Therapy for Tissue Regeneration01:21

Stem Cell Therapy for Tissue Regeneration

Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell types that...

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Related Experiment Video

Updated: May 26, 2026

Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers
08:03

Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers

Published on: July 15, 2019

Optimizing Primary Human Salivary Stem/Progenitor Cells for Tissue Engineering Applications.

Thaise C Geremias1, Fabio H B da Costa1,2, Nadia G Mohyuddin3

  • 1Diagnostic and Biomedical Sciences, School of Dentistry, The University of Texas Health Science Center at Houston, Houston, Texas.

Biorxiv : the Preprint Server for Biology
|May 25, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method to grow human salivary stem cells without animal products, crucial for future regenerative therapies for dry mouth. This xeno-free, serum-free process ensures cell purity and viability for clinical applications.

Keywords:
CD44 antigenStem/progenitor cellsregenerationsalivary glandsxerostomia

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Primary Culture of Dental Pulp Stem Cells
03:45

Primary Culture of Dental Pulp Stem Cells

Published on: May 5, 2023

Related Experiment Videos

Last Updated: May 26, 2026

Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers
08:03

Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers

Published on: July 15, 2019

Primary Culture of Dental Pulp Stem Cells
03:45

Primary Culture of Dental Pulp Stem Cells

Published on: May 5, 2023

Area of Science:

  • Regenerative Medicine
  • Stem Cell Biology
  • Biotechnology

Background:

  • Hyposalivation disorders, or dry mouth, significantly impact quality of life.
  • Current treatments are limited, necessitating novel therapeutic approaches.
  • Autologous cell therapies offer a promising avenue for salivary gland regeneration.

Purpose of the Study:

  • To establish a translationally viable, xeno-free, and serum-free platform for human salivary stem/progenitor cell (hS/PC) isolation and expansion.
  • To develop a protocol suitable for regulatory qualification and FDA-approved clinical trials.
  • To ensure the purity, safety, and proliferative potential of expanded hS/PCs for autologous regenerative therapy.

Main Methods:

  • Human parotid gland tissues were sourced from consented surgical patients.
  • Primary hS/PCs were isolated and cultured under animal-component-free conditions.
  • CD44+ cells were enriched using magnetic cell sorting, with purity assessed via cytokeratins 5/14, CD133, and fibroblast markers.

Main Results:

  • Consistent expansion and epithelial morphology of hS/PCs were achieved in serum-free conditions.
  • High CD44 expression (>95%) and negligible contamination by CD133 or fibroblast markers confirmed epithelial purity.
  • Senescence-associated beta-galactosidase assays showed only a minor, passage-dependent increase in senescent cells (5-16%).

Conclusions:

  • A defined, animal-free culture system effectively supports stable expansion of pure, low-passage hS/PCs.
  • The developed protocol is compatible with Good Manufacturing Practice (GMP) standards.
  • This platform is suitable for regulatory qualification and advancement towards first-in-human autologous regenerative therapy trials for hyposalivation.