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Updated: May 27, 2026
07:42
Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis
Published on: November 26, 2015
Integrating single-cell and bulk transcriptomic analyses to explore key lactylation-related genes in benign prostatic
Xiaotong Pang1,2,3, Deyong Zheng4, Rongbin Zhou2
1Department of Urology, The First Affiliated Hospital of Guangxi Medical University, Guangxi Medical University, Guangxi, 530021, China.
Scientific Reports
|May 25, 2026
Summary
Benign prostatic hyperplasia (BPH) involves inflammation and cell growth. This study identifies ANXA2 and IFI27 as key lactylation-related genes, with ANXA2 deficiency potentially driving BPH progression through metabolic and inflammatory changes.
Area of Science:
- Biochemistry
- Molecular Biology
- Urology
Background:
- Benign prostatic hyperplasia (BPH) is a common age-related condition involving chronic inflammation, metabolic issues, and abnormal cell proliferation.
- Protein lactylation, a post-translational modification linked to metabolism, is increasingly recognized in disease pathogenesis, but its role in BPH is unknown.
Purpose of the Study:
- To investigate the role and molecular features of protein lactylation in benign prostatic hyperplasia (BPH).
- To identify key lactylation-related genes and explore their function in BPH pathogenesis using single-cell and bulk RNA sequencing data.
Main Methods:
- Integrated single-cell RNA sequencing (scRNA-seq) and bulk RNA-seq datasets from BPH and normal prostate tissues.
- Employed machine learning algorithms for feature selection to identify candidate lactylation-related genes.
- Utilized the BPH-1 cell line for in vitro functional studies of ANXA2, including siRNA knockdown and proliferation assays.
Main Results:
- Identified ANXA2 and IFI27 as key lactylation-related genes, significantly downregulated in BPH tissues.
- ANXA2 is predominantly expressed in epithelial cells, while IFI27 is found in endothelial cells.
- ANXA2 knockdown in BPH-1 cells altered glycolysis, reduced lactate production, increased IL-6 secretion, and enhanced cell proliferation.
Conclusions:
- This study provides the first single-cell landscape of lactylation-related gene activity in BPH.
- ANXA2 and IFI27 are identified as critical lactylation-related genes in BPH.
- ANXA2 deficiency may contribute to BPH pathogenesis via metabolic and inflammatory alterations, suggesting potential therapeutic targets.
